School of Chemistry & Chemical Engineering, Xuzhou Normal University, 101 Shanghai Road, Xuzhou 221116, PR China.
J Hazard Mater. 2011 Jan 30;185(2-3):1107-14. doi: 10.1016/j.jhazmat.2010.10.019. Epub 2010 Oct 14.
A simple and sensitive microemulsion extraction separation method was developed for the speciation of aluminium in tea samples by spectrofluorimetry. With 8-hydroxyquinoline (8-HQ) as the chelating agent and Triton X-100 Winsor II microemulsion as the extractant, separation of aluminium species in different pH solutions was achieved by microemulsion extraction. The formation of microemulsion, the conditions of extraction and determination of aluminium species were studied. The results showed that, the contents of aluminium species in tea leaves and infusions samples, such as total aluminium, total soluble aluminium, total granular aluminium, inorganic aluminium except Al-F, and (Al-F+Al-org), were obtained successfully under the optimal conditions. The limit of detection was 0.23 μg L(-1) in pH 9.5 solution, and 0.59 μg L(-1) in pH 6.0 solution respectively; the precision (RSD) for 11 replicate measurements of 10 μg L(-1) aluminium was 2.1% in pH 9.5 solution, and 2.8% in pH 6.0 solution respectively; the recoveries for the spiked samples were 96.8-103.5%. The proposed method is simple and efficient, which has been applied to the speciation of aluminium in tea samples with satisfactory results.
建立了一种简单、灵敏的微乳液萃取分光荧光法用于茶叶中铝的形态分析。以 8-羟基喹啉（8-HQ）为络合剂，Triton X-100Winsor II 型微乳液为萃取剂，通过微乳液萃取实现了不同 pH 值溶液中铝形态的分离。研究了微乳液的形成、萃取条件和铝形态的测定条件。结果表明，在最佳条件下，成功地获得了茶叶和浸出液样品中铝的各种形态的含量，如总铝、总可溶铝、总颗粒铝、除 Al-F 以外的无机铝和（Al-F+Al-有机）。在 pH9.5 溶液中的检出限为 0.23μg/L，在 pH6.0 溶液中的检出限为 0.59μg/L；在 pH9.5 溶液中，10μg/L 铝的 11 次重复测量的精密度（RSD）为 2.1%，在 pH6.0 溶液中的精密度（RSD）为 2.8%；加标样品的回收率为 96.8%-103.5%。该方法简单、高效，已应用于茶叶中铝的形态分析，结果令人满意。
