.National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, 76080, Pakistan.
Biol Trace Elem Res. 2011 Dec;144(1-3):205-16. doi: 10.1007/s12011-011-9070-5. Epub 2011 May 15.
A new method is reported for the separation of aluminum ions (Al(3+)) from interfering cations in pharmaceutical and biological samples through solid-phase extraction (SPE) using 2-methyl-8-hydroxyquinoline (8-hydroxyquinaldine) on activated silica. While separated Al(3+) was preconcentrated by cloud point extraction (CPE) using 3,5,7,2'-4'-pentahydroxyflavone (morin) as complexing reagent, the resulting complex was entrapped in nonionic surfactant (Triton X-114) as prior step to its determination by spectrofluorimetry (SPF). The validity of separation/preconcentration of Al(3+) was checked by certified reference material of human hair and standard addition method. The chemical variables affecting the analytical performance of the separation/preconcentration methods were studied and optimized. The enrichment factor and detection limit of Al(3+) for the preconcentration of 10 ml of dialysate solution and acid-digested samples of scalp hair samples were found to be 25 and 0.34 μg/L, respectively. The relative standard deviation for six replicates of standard containing 20 μg/L of Al(3+) was <10%. In all DS, the concentration of Al was >10 μg/L. The level of Al in scalp hair samples of kidney failure patients was higher than healthy controls.
一种新的方法是通过固相萃取（SPE）使用 2-甲基-8-羟基喹啉（8-羟基喹哪啶）在活性硅胶上从药物和生物样品中的干扰阳离子中分离铝离子（Al（3+））。当分离的 Al（3+）通过浊点萃取（CPE）使用 3,5,7,2'-4'-五羟基黄酮（桑色素）作为络合试剂进行预浓缩时，所得络合物被包封在非离子表面活性剂（Triton X-114）中，作为其通过荧光分光光度法（SPF）测定的前一步。通过人发标准物质和标准加入法检查了 Al（3+）分离/预浓缩的有效性。研究和优化了影响分离/预浓缩方法分析性能的化学变量。对于 10ml 透析液溶液和头皮头发样品酸消解样品的预浓缩，Al（3+）的富集因子和检测限分别为 25 和 0.34μg/L。含 20μg/L Al（3+）的标准品六个重复的相对标准偏差<10%。在所有 DS 中，Al 的浓度均>10μg/L。肾衰竭患者头皮头发样本中的 Al 水平高于健康对照者。
