Departments of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS 66506, USA.
Anticancer Res. 2010 Oct;30(10):3927-32.
Cancer cells have reduced capacity for gap junctional inter-cellular communication (GJIC). One feasible approach to reduce growth of cancer cells is to enhance GJIC. This report shows that a second-generation substituted quinoline, PQ7, has anti-tumor effect. Scrape load/dye transfer and colony growth assays were performed to measure GJIC and tumor formation of T47D breast cancer cells. PQ7 at 500 nM induced a 16-fold increase in the GJIC in T47D cells. In addition to an increase in GJIC, a 50% decrease of colony growth was observed with 100 nM of PQ7. PQ7-treated nu/nu mice showed a 100% regression of xenograft tumor growth of T47D cells. The results show that PQ7 has a promising role in exerting anti-tumor activity in human breast cancer cells.
癌细胞的细胞间隙连接通讯（GJIC）能力降低。增强 GJIC 是减少癌细胞生长的一种可行方法。本报告显示，第二代取代喹啉 PQ7 具有抗肿瘤作用。通过划痕负荷/染料转移和集落生长实验来测量 T47D 乳腺癌细胞的 GJIC 和肿瘤形成。500 nM 的 PQ7 诱导 T47D 细胞中的 GJIC 增加 16 倍。除了 GJIC 增加外，用 100 nM 的 PQ7 观察到集落生长减少 50%。PQ7 处理的 nu/nu 小鼠显示 T47D 细胞的异种移植物肿瘤生长完全消退。结果表明，PQ7 在发挥人类乳腺癌细胞的抗肿瘤活性方面具有广阔的前景。
