Department of General, Visceral, and Thoracic Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Anticancer Res. 2010 Oct;30(10):3933-8.
Orthotopic models utilizing orthotopic implantation have been used for developing cancer models of multiple tumor entities. The aim of this study was to evaluate the role of orthotopic injection in establishing a model of esophageal cancer using a human green fluorescent protein (GFP) cell line of human esophageal carcinoma.
Nude mice were orthotopically injected in the abdominal esophagus with stably transfected GFP-PT1590 cells. Tumor progression was examined by fluorescence imaging.
Fifty percent of animals developed extensive peritoneal spread without a distinct primary tumor at the injection site. Continuous and metastatic spread to the liver, lungs, and lymph nodes was also observed. Fluorescence imaging enabled fast and specific visualization of tumor progression without the need for anesthesia. Intraperitoneal and metastatic tumor spread of GFP-PT1590 esophageal carcinoma demonstrated a highly aggressive but heterogeneous behaviour. Although injection of the esophageal carcinoma cell line GFP-PT1590 did not lead to primary esophageal tumor development at the site of injection, 50% of the mice developed extensive peritoneal spread, as well as lymph node and organ metastasis.
The orthotopic cell injection model resulted in peritoneal carcinomatosis of esophageal adenocarcinoma, which could be visualized in real time using fluorescence imaging.
利用原位种植建立的模型已被用于多种肿瘤实体的癌症模型的开发。本研究的目的是评估在使用人食管癌细胞系 GFP 细胞系建立食管癌模型中，原位注射的作用。
将稳定转染 GFP-PT1590 细胞的裸鼠经腹部食管原位注射。通过荧光成像检查肿瘤进展。
50%的动物出现广泛的腹膜扩散，而注射部位无明显原发性肿瘤。还观察到连续转移到肝、肺和淋巴结。荧光成像能够快速、特异性地观察肿瘤进展，而无需麻醉。 GFP-PT1590 食管癌细胞的腹腔内和转移性肿瘤扩散表现出高度侵袭性但异质性的行为。尽管注射 GFP-PT1590 食管癌细胞系并未导致注射部位原发性食管肿瘤的发展，但 50%的小鼠出现广泛的腹膜扩散以及淋巴结和器官转移。
原位细胞注射模型导致了食管腺癌的腹膜癌病，可通过荧光成像实时可视化。
