Hu Tao, Qi Hui, Li Pei, Zhao Guoqiang, Ma Yangcheng, Hao Qianyun, Gao Chunzhi, Zhang Yilin, Wang Chunyao, Yang Meng, Hoffman Robert M, Chen Ping, Dong Ziming
College of Basic Medical Sciences, Zhengzhou University; Collaborative Innovation Center of Henan province for cancer chemoprevention, Zhengzhou, P.R. China Laboratory Animal Center, Zhengzhou University, Zhengzhou, P.R. China.
AntiCancer Biotech (Beijing) Co., Ltd., Beijing, P.R. China.
Anticancer Res. 2015 Sep;35(9):4655-63.
BACKGROUND/AIMS: Esophageal squamous cell carcinoma (ESCC) is a recalcitrant cancer. Mouse models of this disease could be used for discovery of more effective therapy for ESCC.
The green fluorescent protein (GFP)-expressing human esophageal cancer EC1 cell line was established with a lentiviral expression system. Subsequently, nude mice were injected subcutaneously, intracardiac or intravenously, or orthotopically implanted with EC1-GFP cells. Tumor growth and metastasis were examined by fluorescence in vivo imaging or by open fluorescence imaging after autopsy.
Four different mouse xenograft models of ESCC expressing GFP were established. In the subcutaneous model, primary tumor growth was monitored in real-time by whole-body fluorescence imaging. No metastasis was observed in the subcutaneous or surgical orthotopic implantation model. By 55 days after implantation, all mice had developed orthotopic esophageal cancer, but without detectable metastasis. In contrast, experimental metastasis occurred in the intracardiac and intravenous models. In the intravenous injection model, the lung was the sole organ of experimental metastasis. In the intracardiac model, extensive experimental metastases occurred in the bone, brain and lung.
The mouse xenograft models of ESCC developed in the present study can provide a means of discovering more effective therapy of this recalcitrant type of cancer.
背景/目的：食管鳞状细胞癌（ESCC）是一种难治性癌症。该疾病的小鼠模型可用于发现更有效的ESCC治疗方法。
利用慢病毒表达系统建立了表达绿色荧光蛋白（GFP）的人食管癌EC1细胞系。随后，将EC1-GFP细胞皮下注射、心内注射、静脉注射或原位植入裸鼠体内。通过体内荧光成像或尸检后的开放荧光成像检查肿瘤生长和转移情况。
建立了四种不同的表达GFP的ESCC小鼠异种移植模型。在皮下模型中，通过全身荧光成像实时监测原发性肿瘤的生长。在皮下或手术原位植入模型中未观察到转移。植入后55天，所有小鼠均发生原位食管癌，但未检测到转移。相比之下，心内注射和静脉注射模型中发生了实验性转移。在静脉注射模型中，肺是实验性转移的唯一器官。在心内注射模型中，骨、脑和肺均发生了广泛的实验性转移。
本研究建立的ESCC小鼠异种移植模型可为发现这种难治性癌症的更有效治疗方法提供一种手段。
