多种 NDH-1 复合物在蓝藻电子传递网络中的不同作用，通过对 Synechocystis sp. PCC6803 各种 Ndh 缺失突变体中 P700+ 还原的动力学分析揭示。
Distinct roles of multiple NDH-1 complexes in the cyanobacterial electron transport network as revealed by kinetic analysis of P700+ reduction in various Ndh-deficient mutants of Synechocystis sp. strain PCC6803.
机构信息
Plant Biochemistry, Ruhr University Bochum, D-44780 Bochum, Germany.
出版信息
J Bacteriol. 2011 Jan;193(1):292-5. doi: 10.1128/JB.00984-10. Epub 2010 Oct 29.
Abstract
While methyl viologen had only a small effect on P700(+) rereduction kinetics after far-red pulses in KCN-treated wild-type Synechocystis sp. strain PCC6803 and an NdhF3/NdhF4 (NdhF3/F4)-defective mutant, it involved a rather slow P700(+) rereduction in an NdhF1-defective mutant. This strongly indicates that (i) active electron flow from metabolites to plastoquinone is suppressed upon deletion of ndhF1 and (ii) photosystem 1-mediated cyclic electron transport is dependent on NdhF3/F4-type NDH-1 complexes.
摘要
虽然在 KCN 处理的野生型集胞藻 PCC6803 和 NdhF3/NdhF4（NdhF3/F4）缺陷突变体中，甲基紫精对远红光脉冲后 P700(+)再还原动力学只有很小的影响，但在 NdhF1 缺陷突变体中，它涉及到一个相当缓慢的 P700(+)再还原。这强烈表明：（i）在 ndhF1 缺失时，来自代谢物的活性电子流到质体醌被抑制；（ii）光系统 1 介导的循环电子传递依赖于 NdhF3/F4 型 NDH-1 复合物。
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