College of Pharmacy, Keimyung University, 2800 Dalgubeoldaero, Dalseo-Gu, Daegu 704-701, Republic of Korea.
Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
Int J Syst Evol Microbiol. 2011 Oct;61(Pt 10):2342-2347. doi: 10.1099/ijs.0.027573-0. Epub 2010 Oct 29.
Two aerobic, Gram-reaction-negative, golden-yellow pigmented and rod-shaped bacteria, designated strains A5Q-118(T) and A5Q-27, were isolated from an unidentified sea squirt that thrives in the coral reefs off the coast of Okinawa, Japan. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the novel isolates were affiliated with the family 'Flammeovirgaceae' of the phylum Bacteroidetes. Strains A5Q-118(T) and A5Q-27 shared 100 % sequence similarity with each other and showed <92 % similarity with other cultivated members of the family 'Flammeovirgaceae'. The novel isolates were phenotypically and physiologically different from strains described previously. The DNA G+C content was 35.5-36.2 mol%, MK-7 was the major menaquinone and iso-C(15 : 0) and C(16 : 1)ω5c were the major fatty acids. Based on the results of this polyphasic taxonomic study, it was concluded that strains A5Q-118(T) and A5Q-27 represent a novel species in a new genus of the family 'Flammeovirgaceae', for which the name Aureibacter tunicatorum gen. nov., sp. nov. is proposed. Proposal for designation of the Flammeovirgaceae fam. nov. is also presented. The type strain of Aureibacter tunicatorum is A5Q-118(T) ( = KCTC 23232(T) = NBRC 107587(T)).
两株需氧、革兰氏反应阴性、金黄色色素、杆状的细菌，菌株 A5Q-118(T) 和 A5Q-27，从一种在日本冲绳海岸珊瑚礁中茁壮成长的未鉴定海鞘中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，新分离株属于黄杆菌科的拟杆菌门。菌株 A5Q-118(T) 和 A5Q-27 彼此之间的序列相似度为 100%，与黄杆菌科其他已培养成员的相似度<92%。新分离株在表型和生理上与以前描述的菌株不同。DNA G+C 含量为 35.5-36.2 mol%，主要的menaquinone 为 MK-7，主要脂肪酸为 iso-C(15：0)和 C(16：1)ω5c。基于多相分类学研究的结果，得出结论，菌株 A5Q-118(T) 和 A5Q-27 代表了黄杆菌科的一个新属的一个新种，命名为 Aureibacter tunicatorum gen. nov.，sp. nov.。还提出了 Flammeovirgaceae fam. nov.的命名建议。Aureibacter tunicatorum 的模式菌株为 A5Q-118(T)（=KCTC 23232(T)=NBRC 107587(T)）。
