Korea Institute of Ocean Science and Technology, Ansan, 426-744, Republic of Korea.
Curr Microbiol. 2013 Dec;67(6):742-7. doi: 10.1007/s00284-013-0429-z. Epub 2013 Aug 2.
An aerobic, Gram-negative, coccoid to short rod-shaped and non-flagellated marine bacterial strain S354(T) was isolated from seawater of Micronesia. The strain was capable to degrade agar-forming slight depression into agar plate. Growth occurred at a temperature range of 12-44 °C, a pH range of 5-9, and a salinity range of 1-7 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences suggested that S354(T) belongs to the family Flammeovirgaceae. The novel strain was most closely related to Limibacter armeniacum YM 11-185(T) with similarity of 92.5 %. The DNA G+C content was 43.8 mol%. The major fatty acids (>10 %) were iso-C15:0 and C16:1 ω5c. The predominant isoprenoid quinone was determined to be MK-7. Polar lipid profile of S354(T) consisted of phosphatidylethanolamine, unknown polar lipid, and unknown glycolipids. Based on the phenotypic, phylogenetic, biochemical, and physiological tests conducted in this study, S354(T) is proposed to represent a type strain of a novel genus and species. The 16S rRNA gene sequence of S354(T) is registered in GenBank under the accession number JQ639084. The type of strain Algivirga pacifica gen. nov., sp. nov. is S354(T) (=KCCM 90107(T)=JCM 18326(T)).
一株好氧、革兰氏阴性、球形到短杆状且无鞭毛的海洋细菌菌株 S354(T)从密克罗尼西亚的海水中分离得到。该菌株能够将琼脂形成的轻微凹陷降解到琼脂平板上。生长发生在温度范围为 12-44°C、pH 值范围为 5-9 和盐度范围为 1-7%（w/v）NaCl 的条件下。基于 16S rRNA 基因序列的系统发育分析表明，S354(T)属于 Flammeovirgaceae 科。该新菌株与 Limibacter armeniacum YM 11-185(T)最为密切相关，相似度为 92.5%。该菌株的 DNA G+C 含量为 43.8 mol%。主要脂肪酸（>10%）为 iso-C15:0 和 C16:1 ω5c。主要的异戊烯醌为 MK-7。S354(T)的极性脂谱由磷脂酰乙醇胺、未知极性脂和未知糖脂组成。根据本研究进行的表型、系统发育、生化和生理测试，S354(T)被提议代表一个新属和种的模式菌株。S354(T)的 16S rRNA 基因序列已在 GenBank 中注册，登录号为 JQ639084。Algivirga pacifica 属，种。nov. 的模式菌株是 S354(T)=KCCM 90107(T)=JCM 18326(T)。
