Department of Veterinary Clinics, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Largo Prof. Abel Salazar, 2, 4099-003 Porto, Portugal.
Curr Genomics. 2010 May;11(3):212-20. doi: 10.2174/138920210791110933.
TWIST1 is thought to be a novel oncogene. Understanding the molecular mechanisms regulating the TWIST1 gene expression profiles in tumor cells may give new insights regarding prognostic factors and novel therapeutic targets in veterinary oncology. In the present study we partially isolated the TWIST1 gene in Felis catus and performed comparative studies. Several primer combinations were used based on the alignments of homologous DNA sequences. After PCR amplification, three bands were obtained, purified and sequenced. Several bioinformatic tools were utilized to carry out the comparative studies. Higher similarity was found between the isolated TWIST1 gene in Felis catus and Homo sapiens (86%) than between Homo sapiens and Rattus norvegicus or Mus musculus (75%). Partial amino acid sequence showed no change in the four species analyzed. This confirmed that coding sequences presented high similarity (~96%) between man and cat. These results give the first insights regarding the TWIST1 gene in cat but further studies are required in order to establish, or not, its role in tumor formation and progression in veterinary oncology.
TWIST1 被认为是一个新的癌基因。了解 TWIST1 基因在肿瘤细胞中表达谱的调控分子机制，可能为兽医肿瘤学中的预后因素和新的治疗靶点提供新的见解。在本研究中，我们部分分离了猫的 TWIST1 基因，并进行了比较研究。根据同源 DNA 序列的比对，使用了几种引物组合。经过 PCR 扩增，得到了三个条带，纯化并测序。利用几种生物信息学工具进行了比较研究。在猫中分离的 TWIST1 基因与智人（86%）的相似度高于智人与大鼠或小鼠（75%）的相似度。分析的四个物种的部分氨基酸序列没有变化。这证实了编码序列在人和猫之间具有很高的相似度（约 96%）。这些结果首次揭示了猫 TWIST1 基因的情况，但需要进一步研究以确定其在兽医肿瘤学中的肿瘤形成和进展中的作用。
