Mustafaev M I, Norimov A Sh
Institute of Immunology, Ministry of Health of the USSR, Moscow.
Biomed Sci. 1990 Mar;1(3):274-8.
The mechanism of interaction of the copolymers N-vinylpyrrolidone-acrylic acid and N-vinylpyrrolidone-maleic anhydride with bovine serum albumin, influenza virus total surface antigen (haemagglutinin and neuraminidase), and the BCG protein fraction in the presence of divalent copper ions was investigated. Novel water-soluble triple polymer-metal complexes of the above protein antigens were formed. These complexes showed high immunogenicity and conferred high levels of immunological protection. Study of the replication of pathogenic influenza A virus in animal lungs showed that, in mice immunised with the triple complex containing surface glycoprotein influenza virus A antigens, reproduction of the homologous virus was sharply inhibited, and immunisation of B mice, exhibiting pronounced T-cell deficiency, with complexes containing the BCG protein fraction ensured development of a high level of protection with respect to BCG infection.
研究了共聚物N-乙烯基吡咯烷酮-丙烯酸和N-乙烯基吡咯烷酮-马来酸酐在二价铜离子存在下与牛血清白蛋白、流感病毒全表面抗原（血凝素和神经氨酸酶）以及卡介苗蛋白组分的相互作用机制。形成了上述蛋白质抗原的新型水溶性三元聚合物-金属络合物。这些络合物表现出高免疫原性并赋予高水平的免疫保护。对致病性甲型流感病毒在动物肺部复制的研究表明，在用含有甲型流感病毒表面糖蛋白抗原的三元络合物免疫的小鼠中，同源病毒的繁殖受到显著抑制，而用含有卡介苗蛋白组分的络合物免疫表现出明显T细胞缺陷的B小鼠，可确保对卡介苗感染产生高水平的保护。
