Ma Xiu-Rui, Duan Jun-Li, Zuo Lin, Wang Jin, He Zhong-Mei, Yan Zi, Zheng Rong-Hua, Yang Guang-Zhao, Liu Hui-Rong
Department of Physiology, State Key Laboratory of Cellular Physiology, Shanxi Medical University, Taiyuan 030001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2010 Aug;26(3):257-60.
To investigate the distribution characteristics of autoantibody against beta1 adrenergic receptor (beta1 AR) in the sera of arrhythmia patients and whether the autoantibody could induce arrhythmia.
Healthy subjects and patients with arrhythmia or coronary artery disease were chosen. The autoantibody against beta1 AR in the sera was screened by enzyme-linked immunosorbent assay (ELISA). IgG in the positive autoantibody sera from arrhythmia patients were purified and administrated to normal rats; then the ECGs were dynamic monitored.
The positive rate of autoantibody against beta1 AR in arrhythmia patients was 52.8%, which was significantly higher than that in coronary heart disease group (24%, P < 0.01) and healthy people group (5%, P < 0.01), respectively. Moreover, the autoantibody against beta1 AR could lead to the occurring of arrhythmia in normal rats, most of which were ventricular arrhythmia.
In the sera of arrhythmia patients, the autoantibody against beta1 AR has a high titer and it could lead to the arrhythmia of rats in vivo.
探讨心律失常患者血清中β1肾上腺素能受体（β1AR）自身抗体的分布特征，以及该自身抗体是否能诱发心律失常。
选取健康受试者、心律失常患者或冠心病患者。采用酶联免疫吸附测定（ELISA）法筛选血清中抗β1AR自身抗体。将心律失常患者阳性自身抗体血清中的IgG纯化后给予正常大鼠；然后动态监测心电图。
心律失常患者抗β1AR自身抗体阳性率为52.8%，分别显著高于冠心病组（24%，P<0.01）和健康人组（5%，P<0.01）。此外，抗β1AR自身抗体可导致正常大鼠发生心律失常，其中大多数为室性心律失常。
在心律失常患者血清中，抗β1AR自身抗体滴度较高，且能在体内导致大鼠心律失常。
