使用薄荷油增强治疗神经退行性疾病的治疗剂的鼻脑递送。
Enhancement of nose-brain delivery of therapeutic agents for treating neurodegenerative diseases using peppermint oil.
作者信息
Vaka S R Kiran, Murthy S Narasimha
机构信息
Department of Pharmaceutics, The University of Mississippi, University, MS 38677, USA.
出版信息
Pharmazie. 2010 Sep;65(9):690-2.
The nose-brain pathway is a potential route for drug delivery as it bypasses the brain barriers. The main objective of this study was to investigate the efficacy of peppermint oil in enhancing the bioavailability of intranasally administered neurotrophins like nerve growth factor (NGF). The effect of different concentrations of peppermint oil (PO) on the delivery of NGF across bovine olfactory epithelium was studied in vitro using Franz diffusion cells. Trans-olfactory epithelial electrical resistance (TEER) was measured to assess the permeability status of the bovine olfactory epithelium. The bioavailability of intranasally administered formulations in rat hippocampus was studied by carrying out brain microdialysis in male Sprague-Dawley rats. Peppermint oil at concentrations of 0.05, 0.1 and 0.5% v/v enhanced the in vitro transport of NGF by 5, 7 and 8 fold, respectively. In vivo studies employing brain microdialysis in rats demonstrated that intranasal administration of NGF formulation with 0.5% PO enhanced the bioavailability by approximately 8 fold compared to rats administered with NGF alone. The bioavailability of NGF in the brain could be enhanced by intranasal administration of peppermint oil.
鼻脑通路是一种潜在的给药途径，因为它绕过了脑屏障。本研究的主要目的是研究薄荷油在提高经鼻给药的神经营养因子（如神经生长因子，NGF）生物利用度方面的功效。使用Franz扩散池在体外研究了不同浓度薄荷油（PO）对NGF跨牛嗅上皮转运的影响。测量经嗅觉上皮电阻（TEER）以评估牛嗅上皮的通透性状态。通过对雄性Sprague-Dawley大鼠进行脑微透析，研究了经鼻给药制剂在大鼠海马体中的生物利用度。浓度为0.05%、0.1%和0.5%（v/v）的薄荷油分别使NGF的体外转运提高了5倍、7倍和8倍。在大鼠中采用脑微透析的体内研究表明，与单独给予NGF的大鼠相比，给予含0.5%PO的NGF制剂经鼻给药可使生物利用度提高约8倍。经鼻给予薄荷油可提高NGF在脑中的生物利用度。
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