Hien Tran Thi, Kim Nak Doo, Kim Hyung Sik, Kang Keon Wook
BK21 Project Team, College of Pharmacy, Chosun University, Gwangju, South Korea.
Pharmazie. 2010 Sep;65(9):699-701.
Ginsenoside Rg3 (Rg3), one of the most effective ginseng saponins, has anti-inflammatory and anti-cancer effects. This study examined the effects of Rg3 on cytokine-induced expression of adhesion molecules, which is a key early event in atherogenesis. Rg3 treatment inhibited tumor necrosis factor-alpha (TNF-alpha)-induced protein and mRNA expression of two cell adhesion molecules, vascular cell adhesion molecule-1 (VCAM-1) and intercellular cell adhesion molecule-1 (ICAM-1) in ECV 304 human endothelial cells. In addition, expression of two pro-inflammatory cytokines, TNF-alpha and interleukin-1beta (IL-1beta), was suppressed by Rg3. Reporter gene analyses revealed that minimal reporter activities of nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1) were blocked by Rg3 in a concentration-dependent manner. Taken together, these results indicate that Rg3 may have anti-inflammatory and anti-atherosclerotic activities in the vasculature, which is mediated partly by down-regulation of the expression of cell adhesion molecules and proinflammatory cytokines in endothelial cells.
人参皂苷Rg3（Rg3）是最有效的人参皂苷之一，具有抗炎和抗癌作用。本研究检测了Rg3对细胞因子诱导的黏附分子表达的影响，这是动脉粥样硬化发生过程中的一个关键早期事件。Rg3处理抑制了肿瘤坏死因子-α（TNF-α）诱导的ECV 304人内皮细胞中两种细胞黏附分子，即血管细胞黏附分子-1（VCAM-1）和细胞间黏附分子-1（ICAM-1）的蛋白和mRNA表达。此外，Rg3抑制了两种促炎细胞因子TNF-α和白细胞介素-1β（IL-1β）的表达。报告基因分析显示，Rg3以浓度依赖的方式阻断了核因子-κB（NF-κB）和激活蛋白-1（AP-1）的最小报告基因活性。综上所述，这些结果表明Rg3可能在血管系统中具有抗炎和抗动脉粥样硬化活性，这部分是通过下调内皮细胞中细胞黏附分子和促炎细胞因子的表达来介导的。
