Institute of High Pressure Physics, Polish Academy of Sciences, Sokolowska 29/37, 01-142 Warsaw, Poland.
Langmuir. 2010 Dec 7;26(23):18303-7. doi: 10.1021/la103433g. Epub 2010 Nov 1.
Nanostructuring of noble metal surfaces with biomorphic and biological templates facilitates a variety of applications of surface enhanced Raman scattering (SERS). Here we show that the newly reported insulin amyloid superstructures may be employed as stable nanoscaffolds for metallic Au films providing an effective substrate for SERS on covalently bound molecules of 4-mercaptobenzoic acid (4-MBA). The vortex-aligned insulin fibrils are capable of templating nanopatterns in sputtered Au layers without overlapping the SERS spectra of 4-MBA with vibrational bands stemming from the protein. This holds true regardless of whether the incident laser beam is directly backscattered from the 4-MBA layer, or after passage through the insulin amyloid layer.
利用具有生物形态和生物特性的模板对贵金属表面进行纳米结构化，有利于实现表面增强拉曼散射（SERS）的多种应用。在这里，我们表明，新报道的胰岛素原纤维超结构可用作金属 Au 薄膜的稳定纳米支架，为通过共价键结合的 4-巯基苯甲酸（4-MBA）分子提供 SERS 的有效基底。涡旋排列的胰岛素原纤维能够在溅射 Au 层中形成纳米图案，而不会使 4-MBA 的拉曼光谱与源自蛋白质的振动带重叠。无论入射激光束是直接从 4-MBA 层反向散射，还是经过胰岛素原纤维层后再反向散射，情况均如此。
