King's College London School of Medicine, UK.
Clin Endocrinol (Oxf). 2011 Jan;74(1):67-72. doi: 10.1111/j.1365-2265.2010.03886.x.
Gut hormones peptide YY (PYY) and glucagon-like peptide-1 (GLP-1) play an integral role in appetite control and energy homeostasis. Entero-endocrine L-cells can be stimulated by nutrients and or bile acids to co-secrete PYY and GLP-1. The aim of this study was to determine the response of bile acids, PYY, GLP-1 and ghrelin after a test meal.
Twelve subjects with a BMI of 22·8 (0·52) kg/m² [mean (SEM)] received a 400 kcal test meal after which blood samples were taken every 30 min from 0 to 180 min. PYY, GLP-1 and ghrelin were measured by radioimmunoassays. Fractionated bile acids were measured by HPLC-MSMS.
PYY positively correlated with glycochenodeoxycholic acid (GCDCA) (rs = 0·23, P = 0·03) and taurochenodeoxycholic acid (TCDCA) (rs = 0·26, P = 0·02). GLP-1 positively correlated with GCDCA (rs = 0·22, P = 0·047) and glycodeoxycholic acid (GDCA) (rs = 0·3, P = 0·005). Ghrelin negatively correlated with GDCA (rs = -0·45, P≤ 0·0001), TCDCA (rs = -0·23, P = 0·034) and taurodeoxycholic acid (TDCA) (rs = -0·44, P≤ 0·0001).
PYY and GLP-1 responses correlated with chenodeoxycholic acid (CDCA) counterparts, whereas ghrelin negatively correlated with deoxycholic acid (DCA) counterparts. Specific bile acids may thus differentially affect entero-endocrine cells.
肠促胰素肽 YY（PYY）和胰高血糖素样肽-1（GLP-1）在控制食欲和能量平衡方面发挥着重要作用。肠内分泌 L 细胞可以被营养素和/或胆汁酸刺激而共同分泌 PYY 和 GLP-1。本研究旨在确定餐后胆汁酸、PYY、GLP-1 和 ghrelin 的反应。
12 名 BMI 为 22.8（0.52）kg/m²[平均值（SEM）]的受试者接受 400 卡路里的测试餐，之后在 0 至 180 分钟每隔 30 分钟采集一次血样。用放射免疫分析法测量 PYY、GLP-1 和 ghrelin。用 HPLC-MSMS 测量胆汁酸的分级。
PYY 与甘氨胆酸（GCDCA）呈正相关（rs=0.23，P=0.03）和牛磺胆酸（TCDCA）（rs=0.26，P=0.02）。GLP-1 与 GCDCA 呈正相关（rs=0.22，P=0.047）和甘氨脱氧胆酸（GDCA）（rs=0.3，P=0.005）。ghrelin 与 GDCA 呈负相关（rs=-0.45，P≤0.0001）、TCDCA（rs=-0.23，P=0.034）和牛磺脱氧胆酸（TDCA）（rs=-0.44，P≤0.0001）。
PYY 和 GLP-1 的反应与鹅脱氧胆酸（CDCA）相对应，而 ghrelin 与脱氧胆酸（DCA）相对应呈负相关。特定的胆汁酸可能因此而对肠内分泌细胞产生不同的影响。
