Department of Medical Biochemistry, Faculty of Medicine, Gazi University, Besevler, Ankara, Turkey.
Mycoses. 2011 Sep;54(5):e463-7. doi: 10.1111/j.1439-0507.2010.01951.x. Epub 2010 Oct 29.
Vulvovaginal candidiasis is one of the most frequent disorders in obstetrics and gynaecology. Approximately three-quarters of all adult women experience at least one episode of vulvovaginal candidiasis during their life span. Diabetes mellitus (DM) increases the rate of vaginal colonisation and infection with Candida species. The secreted acid proteinase might be especially relevant in the pathogenesis of vulvovaginal candidiasis. The aim of this study was to determine the acid proteinase activity in the samples of Candida albicans from diabetic patients with vulvovaginal candidiasis by a fluorometric method. Vaginal swabs were taken from 33 women (aged between 22 and 57 years) having symptoms of vaginitis. Patients were divided into three groups: control group, controlled diabetic group and uncontrolled diabetic group. The proteinase activity in the culture supernatants was determined by a modified fluorometric method. Acid proteinase activities were significantly increased in the uncontrolled diabetic group in comparison with both the control group and the controlled diabetic group (P < 0.05). Acid proteinase may play an important role in C. albicans pathogenesis in diabetic patients. Improving glucose control may reduce the risk of Candida colonisation and potentially symptomatic infection, among women with diabetes and hence may be useful even for weaker enzyme activity measurements.
外阴阴道假丝酵母菌病是妇产科最常见的疾病之一。大约四分之三的成年女性在其一生中至少会经历一次外阴阴道假丝酵母菌病。糖尿病（DM）增加了阴道定植和感染念珠菌的几率。分泌的酸性蛋白酶可能在外阴阴道假丝酵母菌病的发病机制中特别重要。本研究旨在通过荧光法测定糖尿病合并外阴阴道假丝酵母菌病患者的白色念珠菌样本中的酸性蛋白酶活性。从 33 名（年龄 22-57 岁）有阴道炎症状的女性中采集阴道拭子。患者分为三组：对照组、控制良好的糖尿病组和控制不佳的糖尿病组。通过改良荧光法测定培养上清液中的蛋白酶活性。与对照组和控制良好的糖尿病组相比，控制不佳的糖尿病组的酸性蛋白酶活性显著升高（P<0.05）。酸性蛋白酶可能在外阴阴道假丝酵母菌病患者白色念珠菌的发病机制中起重要作用。改善血糖控制可能会降低糖尿病女性念珠菌定植和潜在症状性感染的风险，因此，即使是酶活性较弱的测量也可能有用。
