Fatahinia M, Halvaeezadeh M, Rezaei-Matehkolaei A
Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapour University of Medical Sciences, Ahvaz, Iran; Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
J Mycol Med. 2017 Jun;27(2):188-194. doi: 10.1016/j.mycmed.2017.01.009. Epub 2017 Feb 21.
Comparing the activities of secreted enzymes in different fungal species can improve our understanding of their pathogenic role. Secretion of various enzymes by Candida species has been considered for determination of their virulence in different Candida infections including vulvovaginitis.
The aim of this study was to determine and compare the activity of secreted enzymes in Candidia strains isolated from women suspected to vulvovaginal candidiasis (VVC) and referred to some health centers in Khuzestan, Southwestern Iran.
The vaginal secretion samples were taken by swap from 250 suspected women with symptoms of vulvovaginal candidiasis and cultured on CHROMagar Candida medium. Identification of the isolated Candida from culture positive samples performed by the color of colonies and some standard mycological procedures. Activities of phospholipase, hemolysin-α, hemolysin-β, esterase and proteinase were measured in vitro by standard laboratory protocols. The enzymatic activity index (EAI) was calculated for each enzyme in accordance to relevant protocols.
Totally in eighty cases (32%), a Candida strain was isolated which found to be as 52 (65%) Candida albicans; 12 (15%) C. glabrata; 10 (12.5%) C. dubliniensis; 4 (5%) C. krusei, C. tropicalis and C. parapsilosis species (each=1; 1.3%). Among C. albicans strains, 89.1% produced all studied enzymes, while 86% of C. glabrata strains failed to produce proteinase and phospholipase. The EAIs in decreasing order were as hemolysin-β=0.2895, hemolysin-α=0.5420, esterase=0.5753, proteinase=0.7413, and phospholipase=0.7446, respectively. Activity of phospholipase, esterase and proteinase secreted by C. albicans and C. dubliniensis were significantly more than those released by C. glabrata and C. krusei, while 86% of C. glabrata strains did not show esterase activity. On the other hand, the activity rates of hemolysin α and β among all studied isolates were almost similar.
In the present study, the prevalence of VVC among investigated women was higher than the previous report from Khuzestan but C. albicans has yet remained the predominant agent of VVC in this area. Given to the EAI, the virulence of C. albicans in VVC can be mediated by phospholipase, esterase and proteinases.
比较不同真菌物种中分泌酶的活性有助于我们更好地理解它们的致病作用。念珠菌属分泌的各种酶已被用于确定其在包括外阴阴道炎在内的不同念珠菌感染中的毒力。
本研究旨在测定并比较从疑似外阴阴道念珠菌病（VVC）并转诊至伊朗西南部胡齐斯坦省一些医疗中心的女性中分离出的念珠菌菌株分泌酶的活性。
通过棉签采集250名有外阴阴道念珠菌病症状的疑似女性的阴道分泌物样本，并接种于科玛嘉念珠菌培养基上。根据菌落颜色和一些标准真菌学程序对培养阳性样本中分离出的念珠菌进行鉴定。采用标准实验室方案在体外测定磷脂酶、溶血素-α、溶血素-β、酯酶和蛋白酶的活性。根据相关方案计算每种酶的酶活性指数（EAI）。
总共在80例（32%）中分离出念珠菌菌株，其中52例（65%）为白色念珠菌；12例（15%）为光滑念珠菌；10例（12.5%）为都柏林念珠菌；4例（5%）为克鲁斯念珠菌、热带念珠菌和近平滑念珠菌（各1例；1.3%）。在白色念珠菌菌株中，89.1%产生了所有研究的酶，而86%的光滑念珠菌菌株未产生蛋白酶和磷脂酶。EAI从高到低依次为溶血素-β=0.2895、溶血素-α=0.5420、酯酶=0.5753、蛋白酶=0.7413和磷脂酶=0.7446。白色念珠菌和都柏林念珠菌分泌的磷脂酶、酯酶和蛋白酶活性显著高于光滑念珠菌和克鲁斯念珠菌，而86%的光滑念珠菌菌株未显示酯酶活性。另一方面，所有研究分离株中溶血素α和β的活性率几乎相似。
在本研究中，被调查女性中VVC的患病率高于胡齐斯坦省先前的报告，但白色念珠菌仍是该地区VVC的主要病原体。鉴于EAI，白色念珠菌在VVC中的毒力可能由磷脂酶、酯酶和蛋白酶介导。
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