School of Life Sciences and Immune Synapse Research Center, Gwangju Institute of Science and Technology, 1 Oryong-dong, Puk-ku, Gwangju 500-712, Republic of Korea.
J Ethnopharmacol. 2011 Jan 27;133(2):654-62. doi: 10.1016/j.jep.2010.10.054. Epub 2010 Oct 30.
Placenta extract features as a composition of ointments used for skin beautification, dermatological diseases and skin dryness. However, little evidence has been cited about its underlying mechanisms of action by which it exerts a beneficial role in dermatological diseases in vivo. In this study, we intended to test the effect of topical application of porcine placenta extract in mouse model of contact hypersensitivity and elucidate its mechanism of action.
To test the in vitro effect of porcine placenta extract, RAW 264.7 cells were cocultured with porcine placenta extract and stimulated with LPS (1 μg/ml) and the expression of inflammatory mediator TNF-α was estimated by RT-PCR at the mRNA level and by intracellular staining at the protein level. To further test in vivo efficacy, porcine placenta extract was topically applied to the mice with experimental skin hypersensitivity. For in vivo studies placenta extract in gel form was topically applied to ear of DNCB-induced contact hypersensitivity mouse model everyday for 2 weeks and progression of the disease was estimated by following criteria: (a) ear thickness, (b) serum IgE level by ELISA, (c) histological examination of ear tissue by H&E staining and (d) cytokine profile of total cells and CD4(+) T cells by real time PCR.
Topical application of porcine placenta extract on mouse ears with contact hypersensitivity decreased the severity and progression of the disease manifested by reducing ear swelling, inflammation and edema. Histological evaluation showed that placenta extract treatment reduced lymphocyte infiltration in the ear tissues. Protective effect of placenta extract is also associated with down-regulation of serum IgE level and inflammatory cytokine production (IL-1β, IFN-γ, TNF-α, IL-4, IL-12 and IL-17) in total lymph node cells and CD4(+) T cells.
Our data indicate that protective effect of porcine placenta extract in contact hypersensitivity is mediated by inhibition of the inflammatory responses and IgE production, suggesting a potential therapeutic application of porcine placenta extract to modulate skin inflammation.
胎盘提取物作为一种软膏的成分，用于皮肤美容、皮肤病和皮肤干燥。然而，关于其在体内发挥皮肤病有益作用的潜在作用机制，几乎没有引用任何证据。在这项研究中，我们旨在测试猪胎盘提取物在接触性超敏反应小鼠模型中的局部应用效果，并阐明其作用机制。
为了测试猪胎盘提取物的体外效果，将 RAW 264.7 细胞与猪胎盘提取物共培养，并在 LPS（1μg/ml）刺激下，通过 RT-PCR 在 mRNA 水平和细胞内染色在蛋白质水平上评估炎症介质 TNF-α的表达。为了进一步测试体内疗效，将猪胎盘提取物局部应用于实验性皮肤过敏的小鼠。对于体内研究，将凝胶形式的胎盘提取物每天局部应用于 DNCB 诱导的接触超敏反应小鼠模型的耳朵上，持续 2 周，并根据以下标准评估疾病的进展：（a）耳朵厚度，（b）通过 ELISA 测量血清 IgE 水平，（c）通过 H&E 染色对耳组织进行组织学检查，（d）通过实时 PCR 对总细胞和 CD4+T 细胞的细胞因子谱进行分析。
局部应用猪胎盘提取物可减轻接触超敏小鼠耳朵的疾病严重程度和进展，表现为减少耳朵肿胀、炎症和水肿。组织学评估表明，胎盘提取物治疗可减少耳组织中的淋巴细胞浸润。胎盘提取物的保护作用还与下调血清 IgE 水平和炎症细胞因子（IL-1β、IFN-γ、TNF-α、IL-4、IL-12 和 IL-17）的产生有关。
我们的数据表明，猪胎盘提取物在接触性超敏反应中的保护作用是通过抑制炎症反应和 IgE 产生介导的，这表明猪胎盘提取物具有调节皮肤炎症的潜在治疗应用。
