Department of Microbiology and Immunology, Dartmouth Medical School, Lebanon, NH 03756, United States.
Cell Immunol. 2011;266(2):130-4. doi: 10.1016/j.cellimm.2010.09.008. Epub 2010 Oct 30.
Vaccinia virus infection can confer immunity to smallpox by inducing potent T cell and antibody responses. While the CD8 T cell response to vaccinia virus has been well characterized, less is known about factors required for priming and memory for the CD4 T cells. Focusing on two recently described epitopes, we show that after intranasal infection, both I1L and L4R epitopes are co-dominant during the acute response, but the I1L epitope dominates during memory. CD4 T cell priming was intact in the absence of CD80/86, however secondary responses were reduced. This contrasts with our previous data showing CD80/86-CD28 interaction is required for optimal primary and memory CD8 T cell responses. The absence of CD80/86 also changed the immunodominance hierarchy during memory, with the I1L and L4R responses becoming co-dominant in knockout mice. These data highlight different costimulatory requirements for primary CD4 and CD8 T cell responses to vaccinia virus.
牛痘病毒感染可通过诱导有效的 T 细胞和抗体应答来提供对天花的免疫力。虽然已很好地描述了针对牛痘病毒的 CD8 T 细胞应答，但对于启动和记忆 CD4 T 细胞所需的因素知之甚少。我们专注于最近描述的两个表位，结果表明，在鼻内感染后，I1L 和 L4R 表位在急性反应期间都是共显性的，但在记忆中 I1L 表位占主导地位。尽管缺乏 CD80/86，但 CD4 T 细胞的初始阶段仍然完整，但是二次应答减少了。这与我们之前的数据形成对比，该数据表明 CD80/86-CD28 相互作用是最佳原发性和记忆性 CD8 T 细胞应答所必需的。缺乏 CD80/86 也改变了记忆期间的免疫优势等级，I1L 和 L4R 应答在敲除小鼠中变得共显性。这些数据突出了针对牛痘病毒的原发性 CD4 和 CD8 T 细胞应答的不同共刺激需求。
