Oncology and Molecular Pathology Group, Pathology Department, Institut de Recerca Hospital Vall d'Hebron, 08035 Barcelona, Spain.
Hum Pathol. 2011 Feb;42(2):194-203. doi: 10.1016/j.humpath.2010.04.020. Epub 2010 Oct 30.
Previous work from our laboratory has demonstrated that the expression of the ribosomal protein Rplp1 immortalizes primary cells and is involved in transformation. To investigate the role of the P proteins in tumorigenesis, we examined the messenger RNA expression levels of Rplp0, Rplp1, and Rplp2 in a series of 32 patients with gynecologic tumors. The messenger RNA expression level of all 3 P proteins was increased significantly in the tumor tissue, compared with normal tissue. In addition, a total of 140 biopsies of gynecologic cancers (46 endometrioid and 94 ovarian) were investigated. An up-regulation of P protein expression was observed by immunohistochemistry in an average of 27% of the tumors, as compared with normal tissues. Moreover, the level of P protein up-regulation correlated significantly with p53 expression in serous ovarian cancers. This is an important fact because the level of overexpression of the P proteins correlated with the presence of lymph node metastases in serous ovarian cancers. We also observed that endometrial carcinomas that had invaded the myometrium overexpressed P proteins in the invasive front. In addition, we found that the P proteins are up-regulated in a considerable number of patients with the most common types of cancer. Overall, our study shows that P proteins are involved in human cancer and indicates that the expression level of these proteins could be useful as a prognostic marker in specific subtypes of gynecologic tumors.
先前我们实验室的工作表明核糖体蛋白 Rplp1 的表达可使原代细胞永生化，并参与转化。为了研究 P 蛋白在肿瘤发生中的作用，我们检测了 32 名妇科肿瘤患者系列中 Rplp0、Rplp1 和 Rplp2 的信使 RNA 表达水平。与正常组织相比，所有 3 种 P 蛋白在肿瘤组织中的信使 RNA 表达水平均显著增加。此外，我们还对总共 140 份妇科癌症（46 份子宫内膜样和 94 份卵巢）活检进行了研究。免疫组织化学检测发现，与正常组织相比，肿瘤组织中 P 蛋白的表达平均上调了 27%。此外，P 蛋白表达上调的水平与浆液性卵巢癌中 p53 的表达显著相关。这是一个重要的事实，因为 P 蛋白的过度表达水平与浆液性卵巢癌中的淋巴结转移存在相关。我们还观察到，侵袭性较强的子宫内膜癌在侵袭前沿过度表达 P 蛋白。此外，我们发现相当多患有最常见癌症类型的患者的 P 蛋白表达上调。总的来说，我们的研究表明 P 蛋白参与了人类癌症，并表明这些蛋白的表达水平可作为特定类型妇科肿瘤的预后标志物。
