Regional Center of Animal Selection and Reproduction (CERSYRA) JCCM, Valdepeñas, Spain.
Theriogenology. 2011 Jan 1;75(1):65-72. doi: 10.1016/j.theriogenology.2010.07.011. Epub 2010 Oct 30.
The aim of this work was to study the effect of storage temperature during the transport of ovaries on cleavage and blastocyst rates in Iberian red deer, because wild populations of this subspecies are usually far from laboratories. A total of 472 ovaries from 236 Iberian hinds were recovered and maintained in saline solution at 5-8 °C or 20-25 °C for 12 h. After storage, aspirated oocytes were matured with FSH/LH or EGF and the developed embryos were cultured with oviduct epithelial cells monolayer (OCM). A higher (P = 0.009) cleavage rate was obtained when the ovaries were stored at 5-8 °C. However, there were no differences between both storage temperatures in relation to the percentage of blastocysts obtained. Considering the management and production systems of Iberian red deer, this study provides important information about the ovary storage temperature during transport with the purpose of assuring an optimal in vitro embryo production.
本研究旨在探讨在运输过程中卵巢储存温度对伊比利亚红鹿卵裂和囊胚率的影响，因为该亚种的野生种群通常远离实验室。从 236 只伊比利亚母鹿中回收并在 5-8°C 或 20-25°C 的盐溶液中保存了 472 个卵巢 12 小时。储存后，用 FSH/LH 或 EGF 对吸出的卵母细胞进行成熟培养，并将发育的胚胎与输卵管上皮细胞单层（OCM）培养。当卵巢在 5-8°C 下储存时，获得了更高的（P = 0.009）卵裂率。然而，在两种储存温度下，获得的囊胚百分比没有差异。考虑到伊比利亚红鹿的管理和生产系统，本研究提供了有关在运输过程中卵巢储存温度的重要信息，目的是确保最佳的体外胚胎生产。
