采用十六烷基三甲基溴化铵（CTAB）以96孔板形式从冻干植物组织中提取DNA。
DNA extraction from freeze-dried plant tissue with CTAB in a 96-well format.
作者信息
Hsia An-Ping, Chen Hsin D, Ohtsu Kazuhiro, Schnable Patrick S
出版信息
Cold Spring Harb Protoc. 2010 Nov 1;2010(11):pdb.prot5516. doi: 10.1101/pdb.prot5516.
PMID:21041389
Abstract
This protocol is a modified version of DNA isolation using cetyltrimethylammonium bromide (CTAB) and 96-well plates. It is high-throughput, which facilitates the analysis of large mapping populations. The DNA yield is adequate for at least 100-500 polymerase chain reaction (PCR) procedures.
摘要
本方案是使用十六烷基三甲基溴化铵（CTAB）和96孔板进行DNA提取的改良版。它具有高通量特点，便于对大型定位群体进行分析。DNA产量足以满足至少100 - 500次聚合酶链反应（PCR）操作。
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