U2AF35 相关蛋白 Urp 与 3' 剪接位点结合，促进 U12 型内含子剪接和 U2 型内含子剪接的第二步。
The U2AF35-related protein Urp contacts the 3' splice site to promote U12-type intron splicing and the second step of U2-type intron splicing.
机构信息
Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea.
出版信息
Genes Dev. 2010 Nov 1;24(21):2389-94. doi: 10.1101/gad.1974810.
Abstract
The U2AF35-related protein Urp has been implicated previously in splicing of the major class of U2-type introns. Here we show that Urp is also required for splicing of the minor class of U12-type introns. Urp is recruited in an ATP-dependent fashion to the U12-type intron 3' splice site, where it promotes formation of spliceosomal complexes. Remarkably, Urp also contacts the 3' splice site of a U2-type intron, but in this case is specifically required for the second step of splicing. Thus, through recognition of a common splicing element, Urp facilitates distinct steps of U2- and U12-type intron splicing.
摘要
先前已有研究表明 U2AF35 相关蛋白 Urp 参与 U2 型内含子的剪接。本文发现 Urp 同样参与 U12 型内含子的剪接。UrP 以依赖于 ATP 的方式被招募到 U12 型内含子 3' 剪接位点，在该处促进剪接体复合物的形成。值得注意的是，UrP 还与 U2 型内含子的 3' 剪接位点结合，但在这种情况下，UrP 特异性地需要参与剪接的第二步。因此，通过识别共同的剪接元件，Urp 促进 U2 型和 U12 型内含子剪接的不同步骤。
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1
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2
An early evolutionary origin for the minor spliceosome.小剪接体的早期进化起源。
Nature. 2006 Oct 19;443(7113):863-6. doi: 10.1038/nature05228.
3
Splicing of a rare class of introns by the U12-dependent spliceosome.由U12依赖型剪接体对一类罕见内含子进行剪接。
Biol Chem. 2005 Aug;386(8):713-24. doi: 10.1515/BC.2005.084.
4
A mutational analysis of U12-dependent splice site dinucleotides.U12依赖型剪接位点二核苷酸的突变分析。
RNA. 2005 Sep;11(9):1430-40. doi: 10.1261/rna.7206305. Epub 2005 Jul 25.
5
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Genes Dev. 2004 Jul 1;18(13):1513-26. doi: 10.1101/gad.1206204.
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7
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