在神经前体细胞簇中的 Notch 信号传导过程中，通过不同的 Suppressor of Hairless DNA 结合位点结构对转录进行差异调控。
Differential regulation of transcription through distinct Suppressor of Hairless DNA binding site architectures during Notch signaling in proneural clusters.
机构信息
Burke Medical Research Institute, 785 Mamaroneck Ave., White Plains, NY 10605, USA.
出版信息
Mol Cell Biol. 2011 Jan;31(1):22-9. doi: 10.1128/MCB.00003-10. Epub 2010 Nov 1.
Abstract
In Drosophila melanogaster, achaete (ac) and m8 are model basic helix-loop-helix activator (bHLH A) and repressor genes, respectively, that have the opposite cell expression pattern in proneural clusters during Notch signaling. Previous studies have shown that activation of m8 transcription in specific cells within proneural clusters by Notch signaling is programmed by a "combinatorial" and "architectural" DNA transcription code containing binding sites for the Su(H) and proneural bHLH A proteins. Here we show the novel result that the ac promoter contains a similar combinatorial code of Su(H) and bHLH A binding sites but contains a different Su(H) site architectural code that does not mediate activation during Notch signaling, thus programming a cell expression pattern opposite that of m8 in proneural clusters.
摘要
在黑腹果蝇中，achaete (ac) 和 m8 分别是模型碱性螺旋-环-螺旋激活子（bHLH A）和阻遏子基因，在 Notch 信号传导过程中，它们在神经前簇中的细胞表达模式相反。先前的研究表明， Notch 信号通过特定的细胞内 m8 转录激活，是由包含 Su(H) 和神经前 bHLH A 蛋白结合位点的“组合”和“结构”DNA 转录代码编程的。在这里，我们展示了一个新的结果，即 ac 启动子包含一个类似的 Su(H)和 bHLH A 结合位点的组合代码，但包含一个不同的 Su(H) 位点结构代码，该代码在 Notch 信号传导过程中不介导激活，从而编程了神经前簇中与 m8 相反的细胞表达模式。
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