Mayer R, Zaghouani H, Usuba O, Bona C
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029.
Autoimmunity. 1990;6(4):293-305. doi: 10.3109/08916939008998421.
Studies presented here demonstrate the expression of the Ly-1 gene and the detection of the Ly-1 cytodifferentiation antigen in murine hybridomas producing autoantibodies. We examined the transcription of the Ly-1 gene in thymocytes and 140 hybridomas producing autoantibodies of various specificities which were obtained from normal and autoimmune disease prone mouse strains. As previously demonstrated thymocytes stain brightly for Ly-1 by immunofluorescence and express Ly-1 transcripts. In our panel of hybridomas producing autoantibodies Ly-1 transcripts were detected in 31 (45%) out of 69 NZB hybridomas and 7 (88%) out of 8 viable motheaten hybridomas. S1 nuclease protection experiments showed that Ly-1 transcripts detected in thymocytes and B cells are the product of the same gene. The B cell transcripts are functional since immunofluorescence and Western data presented here detected the Ly-1 protein in hybridomas cells which were found to transcribe the Ly-1 gene. Interestingly a polymorphic transcription of the Ly-1 gene was observed in B cells and B cell hybridomas as compared to thymocytes. Our results obtained in the hybridoma system firmly establish a major contribution of the Ly-1 B cell subset to the production of DNA specific autoantibodies and a smaller contribution to the production of rheumatoid factors and "natural", multispecific autoantibodies.
本文所呈现的研究证明了Ly - 1基因在产生自身抗体的小鼠杂交瘤中的表达以及Ly - 1细胞分化抗原的检测。我们检测了胸腺细胞以及从正常和易患自身免疫性疾病的小鼠品系中获得的140种产生各种特异性自身抗体的杂交瘤中Ly - 1基因的转录情况。如先前所示，胸腺细胞通过免疫荧光对Ly - 1染色明亮并表达Ly - 1转录本。在我们的产生自身抗体的杂交瘤组中，69种新西兰黑（NZB）杂交瘤中有31种（45%）以及8种存活的肌无力杂交瘤中有7种（88%）检测到了Ly - 1转录本。S1核酸酶保护实验表明，在胸腺细胞和B细胞中检测到的Ly - 1转录本是同一基因的产物。B细胞转录本具有功能，因为本文所呈现的免疫荧光和蛋白质印迹数据在被发现转录Ly - 1基因的杂交瘤细胞中检测到了Ly - 1蛋白。有趣的是，与胸腺细胞相比，在B细胞和B细胞杂交瘤中观察到了Ly - 1基因的多态性转录。我们在杂交瘤系统中获得的结果有力地证实了Ly - 1 B细胞亚群对DNA特异性自身抗体产生的主要贡献以及对类风湿因子和“天然”多特异性自身抗体产生的较小贡献。
