分泌天然自身抗体的杂交瘤中Ly1和V基因表达

Ly1 and V-gene expression among hybridomas secreting natural autoantibody.

作者信息

Kaushik A, Mayer R, Fidanza V, Zaghouani H, Lim A, Bona C, Dighiero G

机构信息

Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029.

出版信息

J Autoimmun. 1990 Dec;3(6):687-700. doi: 10.1016/s0896-8411(05)80036-8.

DOI:10.1016/s0896-8411(05)80036-8

Abstract

This report presents evidence that multispecific natural autoantibodies (NAAb) arise from both Ly-1+ and Ly-1- B lymphocyte subsets based upon detection of mRNA transcript of Ly1 gene by Northern blotting among hybridomas from several mouse strains. Newborn Xid mice also possess B cells transcribing the Ly-1 gene that secrete multispecific NAAbs. Different VH and VK gene families were expressed at random in both Ly-1+ and Ly-1- hybridoma clones secreting NAAbs. Certain VK gene families VK1, -8, -9, -10 and -19, were preferentially used by NAAbs. No particular VH:VK pairings were observed among NAAbs multispecifically reactive with DNA and cytoskeleton proteins. The NAAbs reactive with BrMRBC exhibited predominant pairing between VH11:VK9 gene families, although, for the first time, we noted the participation of VK1, -10 and -19 gene families in encoding this autoantibody specificity.

摘要

本报告提供的证据表明，基于对几种小鼠品系杂交瘤中Ly1基因mRNA转录本的Northern印迹检测，多特异性天然自身抗体（NAAb）产生于Ly-1+和Ly-1- B淋巴细胞亚群。新生Xid小鼠也拥有转录Ly-1基因并分泌多特异性NAAb的B细胞。在分泌NAAb的Ly-1+和Ly-1-杂交瘤克隆中，不同的VH和VK基因家族随机表达。某些VK基因家族VK1、-8、-9、-10和-19被NAAb优先使用。在与DNA和细胞骨架蛋白多特异性反应的NAAb中，未观察到特定的VH:VK配对。与BrMRBC反应的NAAb在VH11:VK9基因家族之间表现出主要配对，不过，我们首次注意到VK1、-10和-19基因家族参与编码这种自身抗体特异性。