Plant regeneration from protoplasts of a super Chinese rice (Oryza sativa L.) cultivar--Zhonghua NO.8.

Green rice plants were regenerated from protoplasts, which were derived from cell suspension of Oryza sativa L. cut. Zhonghua No. 8, a super Chinese rice cultivar with high productivity, good quality and high resistance to both bacterial blight and blast. The embryogenic calli were initiated from mature embroys. It took about 4 months to establish the cell suspenion. The regeneration plants from protoplasts were obtained in 2 months after the isolation of protoplasts.