Li S J, Li X H, Sun Y R
Institute of Genetics, Academia Sinica, Beijing.
Chin J Biotechnol. 1989;5(1):39-46.
Mesophyll protoplasts from commercial potatoes were cultured in shallow liquid media. The regenerated cells divided and formed calli. Regenerant plants of two potato lines (Ke Xin 4; 68-62) were obtained from the regenerated calli after transfer to solid medium. The division frequency of the regenerated cells was influenced by culture protoplast density. Growth of the protoplast-derived colonies was sensitive to sucrose concentration in the liquid medium. The protoplast culture medium obviously affected differentiation frequency for the protoplast-derived calli (P-calli). The P-callus differentiation frequency was raised by supplementing the medium with 3% mannitol, which also shortened the P-callus differentiation.
将市售马铃薯的叶肉原生质体培养于浅层液体培养基中。再生细胞分裂并形成愈伤组织。将再生愈伤组织转移至固体培养基后，获得了两个马铃薯品系（克新4号；68-62）的再生植株。再生细胞的分裂频率受培养原生质体密度的影响。原生质体来源菌落的生长对液体培养基中的蔗糖浓度敏感。原生质体培养基明显影响原生质体来源愈伤组织（P-愈伤组织）的分化频率。通过在培养基中添加3%甘露醇提高了P-愈伤组织的分化频率，这也缩短了P-愈伤组织的分化时间。
