Van Damme J, Bunning R A, Conings R, Graham R, Russell G, Opdenakker G
Rega Institute for Medical Research, University of Leuven, Belgium.
Cytokine. 1990 Mar;2(2):106-11. doi: 10.1016/1043-4666(90)90004-d.
Human articular chondrocytes, when stimulated with interleukin 1 beta (IL 1 beta), tumor necrosis factor-alpha (TNF-alpha), or with the double stranded RNA poly (rI).poly (rC), produce a chemotactic activity for granulocytes. The induction with IL 1 beta could be abolished by an antibody to IL 1 beta but not by an antibody to interleukin 6 (IL 6), indicating that the latter is not a mediator for the production of chemotactic activity. The inducers had no direct chemotactic effect on granulocytes. The granulocyte chemotactic factor from chondrocytes was characterized with a specific antibody against leukocyte-derived interleukin 8 (IL 8). The specificity of this antibody was demonstrated by immunochemical and biological criteria such that it could immunoprecipitate only the 6-7 kDa IL 8 protein from fibroblasts, and that it did not neutralize a structurally related monocyte chemotactic protein. This antibody against IL 8 completely neutralized the granulocyte chemotactic activity from stimulated chondrocytes. This demonstrates the identity of chondrocyte IL 8 with leukocyte- and fibroblast-derived IL 8. Our data show that leukocyte chemotaxis into the inflamed joint can be mediated by IL 8, induced in both synovial fibroblasts and chondrocytes by the inflammatory cytokines IL 1 and TNF-alpha.
人关节软骨细胞在受到白细胞介素1β（IL - 1β）、肿瘤坏死因子 - α（TNF - α）或双链RNA聚肌苷酸 - 聚胞苷酸（poly (rI).poly (rC)）刺激时，会产生对粒细胞的趋化活性。用抗IL - 1β抗体可消除IL - 1β介导的诱导作用，但抗白细胞介素6（IL - 6）抗体则不能，这表明IL - 6不是趋化活性产生的介质。诱导剂对粒细胞没有直接趋化作用。用针对白细胞衍生的白细胞介素8（IL - 8）的特异性抗体对软骨细胞产生的粒细胞趋化因子进行了鉴定。该抗体的特异性通过免疫化学和生物学标准得以证实，即它只能从成纤维细胞中免疫沉淀出6 - 7 kDa的IL - 8蛋白，并且不能中和结构相关的单核细胞趋化蛋白。这种抗IL - 8抗体完全中和了受刺激软骨细胞产生的粒细胞趋化活性。这证明了软骨细胞IL - 8与白细胞和成纤维细胞衍生的IL - 8具有同一性。我们的数据表明，白细胞向炎症关节的趋化作用可由IL - 8介导，IL - 1和TNF - α等炎症细胞因子可在滑膜成纤维细胞和软骨细胞中诱导其产生。
