Poole S, Bird T A, Selkirk S, Gaines-Das R E, Choudry Y, Stephenson S L, Kenny A J, Saklatvaa J
National Institute for Biological Standards and Control, Potters Bar, Herts, UK.
Cytokine. 1990 Nov;2(6):416-22. doi: 10.1016/1043-4666(90)90050-4.
The tissue distribution and route of clearance of human recombinant interleukin 1 alpha (IL 1 alpha) injected intravenously in rats was studied. The plasma half-life was approximately 2.5 min, and this was increased after nephrectomy, the kidney being the major organ through which the IL 1 alpha was excreted. Two iodinated fragments of IL 1 alpha, of approximately 5 and 9 kDa, were excreted by the kidneys whereas only intact, 17-kDa IL 1 alpha was detected in plasma, suggesting that the protein was being degraded after uptake by the kidney. The results of in vivo experiments in which surface endopeptidase-24.11 was inhibited with phosphoramidon and in vitro experiments in which rat kidney homogenates were incubated with radiolabeled IL 1 alpha suggest that the cytokine was endocytosed and then hydrolysed by lysosomal proteinases.
研究了静脉注射给大鼠的重组人白细胞介素1α（IL - 1α）的组织分布和清除途径。血浆半衰期约为2.5分钟，肾切除术后该半衰期延长，肾脏是IL - 1α排泄的主要器官。肾脏排泄出两个分子量约为5 kDa和9 kDa的IL - 1α碘化片段，而血浆中仅检测到完整的17 kDa的IL - 1α，这表明该蛋白在被肾脏摄取后发生降解。用磷酰胺抑制表面内肽酶 - 24.11的体内实验结果以及用放射性标记的IL - 1α孵育大鼠肾脏匀浆的体外实验结果表明，该细胞因子被内吞，然后被溶酶体蛋白酶水解。
