液相色谱法测定药物制剂中盐酸黄酮哌酯的含量。
Liquid Chromatographic Determination of Flavoxate HCl in Pharmaceutical Formulation.
作者信息
Attimarad M
机构信息
Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, KSA.
出版信息
J Young Pharm. 2010 Jul;2(3):280-3. doi: 10.4103/0975-1483.66787.
The objective of the study was to develop a high performance liquid chromatography (HPLC) method using ultra violet (UV) detection for the determination of flavoxate HCl in bulk and solid dosage forms by using ibuprofen as the internal standard. Eclipse C18 column (150 mm × 4.6 mm, 5 μm) was used as the stationary phase with a mixture of acetonitrile : 0.1% formic acid in water (75: 25 v/v) as the mobile phase. The response of the drug was linear in the concentration range of 1 - 250 μg/ml. Limit of detection and Limit of quantification were found to be 0.23 μg/ml and 0.69 μg/ml, respectively. The percentage of recovery ranged between 97.4 and 101.3%. The factors affecting column separation of the analyte were studied. The results demonstrated that this method is reliable, reproducible, and suitable for routine quantitative use.
本研究的目的是开发一种高效液相色谱（HPLC）方法，该方法使用紫外（UV）检测，以布洛芬作为内标物，用于测定原料药和固体剂型中的盐酸黄酮哌酯。使用Eclipse C18柱（150 mm×4.6 mm，5μm）作为固定相，乙腈与0.1%甲酸水溶液（75:25 v/v）的混合物作为流动相。药物的响应在1 - 250μg/ml的浓度范围内呈线性。检测限和定量限分别为0.23μg/ml和0.69μg/ml。回收率在97.4%至101.3%之间。研究了影响分析物柱分离的因素。结果表明，该方法可靠、可重现，适用于常规定量分析。
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