快速反相高效液相色谱法测定片剂中氯诺昔康的含量
Rapid rp HPLC method for quantitative determination of lornoxicam in tablets.
作者信息
Attimarad Mahesh
机构信息
Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, KSA.
出版信息
J Basic Clin Pharm. 2010 Mar;1(2):115-8. Epub 2010 May 15.
The objective of the study was to develop a simple, rapid, specific and precise reverse phase high performance liquid chromatographic method for the determination of lornoxicam in bulk and pharmaceutical preparations. Chromatographic separation of the drug was performed on a eclipse C18 column (150 mm × 4.6 mm, 5 μm) as stationary phase and mobile phase used is methanol: 0.1% formic acid in water (80:20 v/v), with a flow rate of 0.8 mL min(-1) and UV detection at 381 nm. The proposed method was validated for linearity, accuracy, precision, limit of detection (LOD) and limit of quantitation (LOQ). Linearity, accuracy and precision were found to be acceptable over the ranges of 0.5-20 μg/ml. It can be conveniently adopted for routine quality control analysis of lornoxicam.
本研究的目的是开发一种简单、快速、特异且精确的反相高效液相色谱法，用于测定原料药及药物制剂中的氯诺昔康。药物的色谱分离在月旭C18柱（150 mm×4.6 mm，5μm）上进行，以其作为固定相，流动相为甲醇：0.1%甲酸水溶液（80:20 v/v），流速为0.8 mL min(-1)，在381 nm处进行紫外检测。该方法针对线性、准确度、精密度、检测限（LOD）和定量限（LOQ）进行了验证。在0.5 - 20μg/ml范围内，线性、准确度和精密度均可接受。该方法可方便地用于氯诺昔康的常规质量控制分析。
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