Xu Yong-Gang, Yu Wan-Tai, Ma Qiang, Zhou Hua
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
Ying Yong Sheng Tai Xue Bao. 2010 Aug;21(8):2078-85.
Taking a long-term field experiment on an aquic brown soil in the Shenyang Ecological Station of Chinese Academy of Sciences as a platform, the methods of traditional chloroform fumigation and modern PCR-DGGE were adopted to study the effects of different fertilizations on the changes of soil microbial biomass and bacterial community structure. During the whole period of the experiment, there was a similar trend in the dynamic changes of soil microbial biomass C and N. Long-term application of organic manure increased the soil organic C and the soil microbial biomass C and N significantly, while long-term application of chemical fertilizers had significant negative effects on the soil pH and soil microbial biomass C and N. The DGGE patterns showed that under different fertilizations, the majority of soil bacterial 16S rDNA bands were the same, 18 of the 28 bands being common, suggesting that the bacterial populations in test soil were more stable, but their numbers were affected by different fertilizations. Long-term application of organic fertilizer could promote the diversity of the bacterial community structure in aquic brown soil, while the application of chemical fertilizers was in adverse.
以中国科学院沈阳生态站的潮棕壤长期田间试验为平台，采用传统的氯仿熏蒸法和现代的PCR-DGGE法，研究不同施肥方式对土壤微生物生物量和细菌群落结构变化的影响。在整个试验期间，土壤微生物生物量碳和氮的动态变化趋势相似。长期施用有机肥显著增加了土壤有机碳以及土壤微生物生物量碳和氮，而长期施用化肥对土壤pH值以及土壤微生物生物量碳和氮有显著负面影响。DGGE图谱显示，在不同施肥方式下，大多数土壤细菌16S rDNA条带相同，28条带中有18条是共有的，这表明供试土壤中的细菌种群较为稳定，但其数量受不同施肥方式的影响。长期施用有机肥可促进潮棕壤细菌群落结构的多样性，而施用化肥则起相反作用。
