Innovation Center for Medical Redox Navigation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Anal Chem. 2010 Dec 1;82(23):9789-96. doi: 10.1021/ac101998z. Epub 2010 Nov 2.
A sensitive and simultaneous analytical technique for visualizing multiple endogenous molecules is now strongly required in biological science. Here, we show the applicability of a matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) system for getting chemically diverse metabolite profiles on a single-mammalian cell. This ultrahighly sensitive MALDI-MS technique enabled a spatially resolved detection of a broad range of metabolites including nucleotides, cofactors, phosphorylated sugars, amino acids, lipids, and carboxylic acids in normal mouse brain tissue with their unique distributions. Furthermore, a combination of MS imaging and metabolic pathway analysis of a rat transient middle cerebral artery occlusion model visualized a spatiotemporal behavior of metabolites in the central metabolic pathway regulated by an ischemia reperfusion. These findings highlight potential applications of an in situ metabolomic imaging technique to visualize spatiotemporal dynamics of the tissue metabolome, which will facilitate biological discovery in both preclinical and clinical settings.
在生物科学领域，现在强烈需要一种能够灵敏且同时分析多种内源性分子的分析技术。在这里，我们展示了基质辅助激光解吸电离质谱（MALDI-MS）系统在获取单个哺乳动物细胞上化学多样性代谢物图谱方面的适用性。这种超高灵敏度的 MALDI-MS 技术能够在正常小鼠脑组织中实现包括核苷酸、辅助因子、磷酸化糖、氨基酸、脂质和羧酸在内的广泛代谢物的空间分辨检测，并且具有独特的分布。此外，对大鼠短暂性大脑中动脉闭塞模型的 MS 成像和代谢途径分析的结合，可视化了由缺血再灌注调节的中心代谢途径中代谢物的时空行为。这些发现突出了原位代谢组学成像技术在可视化组织代谢组时空动态方面的潜在应用，这将有助于临床前和临床环境中的生物学发现。
