Ames Laboratory, U.S. Department of Energy, and Department of Chemistry, Iowa State University, Ames, Iowa 50011, United States.
J Am Chem Soc. 2010 Nov 24;132(46):16417-22. doi: 10.1021/ja106506k. Epub 2010 Nov 2.
Gold nanorods are excellent orientation probes due to their anisotropic optical properties. Their dynamic rotational motion in the 3D space can be disclosed with Nomarski-type differential interference contrast (DIC) microscopy. We demonstrate that by using the combination of gold nanorod probes and DIC microscopy, we are able to resolve rotational motions of nano-cargos transported by motor proteins at video rate not only on engineered surfaces but also on cytoskeleton tracks in live cells.
金纳米棒具有各向异性的光学特性，是出色的取向探针。通过诺马斯基型微分干涉对比（DIC）显微镜，可以揭示其在三维空间中的动态旋转运动。我们证明，通过将金纳米棒探针与 DIC 显微镜相结合，不仅可以在工程表面上，而且可以在活细胞的细胞骨架轨迹上，以视频速率解析马达蛋白运输的纳米货物的旋转运动。
