Department of Chemical Pathology and Human Metabolism, Royal Free Hospital and School of Medicine, (University of London), Pond Street, London, NW3 2QG, UK.
Platelets. 1995;6(4):200-3. doi: 10.3109/09537109509078455.
Gamma-glutamyltransferase (GGT) activity in human platelet sonicates was 13.6 u/g of protein (range: 7.9-25.0) in 13 healthy, non-smoking, female volunteers; corresponding values in 16 males were: 20.3 (10.1-26.0). These values Mered significantly (p = 0.034). Platelet and serum GGT activity correlated significantly (p > 0.04). Platelets seem to contain only the isoenzyme GGT 4. Part of this enzyme activity is in the form of aggregates or linked with membranes/proteins. This activity is released by Triton X-100 and trypsin and migrates as GGT 4. Serum GGT activity, a measurement in routine use, could be influenced by GGT released by platelets. It is therefore of interest that serum GGT activity can be increased in clinical conditions (e.g. myocardial infarction, diabetes, peripheral vascular disease) associated with platelet hyperactivity. Platelet GGT may influence intracellular S-nitrosoglutathione (a putative nitric oxide donor) levels. Potential associations between serum GGT activity and platelet function indices deserve investigation.
人血小板超声提取物中的γ-谷氨酰转移酶（GGT）活性在 13 名健康、不吸烟的女性志愿者中为 13.6u/g 蛋白（范围：7.9-25.0）；16 名男性的相应值为：20.3（10.1-26.0）。这些值差异显著（p=0.034）。血小板和血清 GGT 活性显著相关（p＞0.04）。血小板似乎只含有同工酶 GGT4。部分酶活性呈聚集状态或与膜/蛋白相连。这种活性可被 Triton X-100 和胰蛋白酶释放，并作为 GGT4 迁移。血清 GGT 活性是常规测量指标，可能会受到血小板释放的 GGT 的影响。因此，与血小板活性过高相关的临床病症（如心肌梗死、糖尿病、外周血管疾病）中血清 GGT 活性升高的情况非常有趣。血小板 GGT 可能会影响细胞内 S-亚硝基谷胱甘肽（一种潜在的一氧化氮供体）水平。值得研究血清 GGT 活性与血小板功能指标之间的潜在关联。
