Thompson, Asthma and Allergy Research Unit, Department of Medicine, University of Western Australia, Queen Elizabeth II Medical Centre, Nedlands, WA, 6009, Australia.
Platelets. 1995;6(6):394-401. doi: 10.3109/09537109509078478.
The effects of varying concentrations of platelet-activating factor (PAF), arachidonic acid (AA) and collagen on the expression of the platelet activation markers CD63 and CD62P were assessed in 10 normal subjects using flow cytometry. CD63 expression was significantly greater than CD62P expression, with PAF (80 nM) inducing mean maximum CD63 expression of 32.9 ± 6.4% and mean maximum CD62P expression of 5.5 ± 1.8%. AA (1 mM) induced maximum CD63 expression of 37.7 ± 7% and maximum CD62P expression of 9.3 ± 1%. Collagen (2-80 pg/ml) induced minimal expression but 800 pg/ml induced mean CD63 expression of 33.1 ± 4.1% and mean CD62P expression of 6.1 ± 0.8%. Greater CD63 and CD62P expression were induced by phorbol myristate acetate (1.6 pM, 70.9 ± 11% and 69.4 ± 9.9%, respectively) and thrombin (0.1 U/ml, 70.7 ± 9.3% and 73.5 ± 5.4%, respectively). With PAF and collagen only one platelet population was detected whereas with 1 mM AA two populations were observed. These results indicate that expression of platelet adhesion receptors depends on the nature and concentration of agonist and that subpopulations of platelets may exist. Importantly, PAF concentrations inducing moderate CD63 and CD62P expression did not induce platelet aggregation, suggesting that platelets can be activated independently of aggregation.
采用流式细胞术评估了不同浓度的血小板激活因子 (PAF)、花生四烯酸 (AA) 和胶原对 10 名正常受试者血小板活化标志物 CD63 和 CD62P 表达的影响。CD63 的表达明显高于 CD62P 的表达，PAF（80 nM）诱导的 CD63 最大表达均值为 32.9±6.4%，CD62P 的最大表达均值为 5.5±1.8%。AA（1 mM）诱导的 CD63 最大表达均值为 37.7±7%，CD62P 的最大表达均值为 9.3±1%。胶原（2-80 pg/ml）诱导的表达最低，但 800 pg/ml 诱导的 CD63 表达均值为 33.1±4.1%，CD62P 的表达均值为 6.1±0.8%。佛波醇肉豆蔻酸酯 (1.6 pM，分别为 70.9±11%和 69.4±9.9%) 和凝血酶 (0.1 U/ml，分别为 70.7±9.3%和 73.5±5.4%) 诱导的 CD63 和 CD62P 表达更高。用 PAF 和胶原仅检测到一个血小板群体，而用 1 mM AA 则观察到两个群体。这些结果表明，血小板黏附受体的表达取决于激动剂的性质和浓度，并且可能存在血小板亚群。重要的是，诱导中度 CD63 和 CD62P 表达的 PAF 浓度不会诱导血小板聚集，这表明血小板可以在不聚集的情况下被激活。
