内源性重组普通狨猴（Callithrix jacchus）促卵泡激素的分子克隆与功能表征
Molecular cloning and functional characterization of endogenous recombinant common marmoset monkey (Callithrix jacchus) follicle-stimulating hormone.
作者信息
Müller T, Hupfeld T, Roessler J, Simoni M, Gromoll J, Behr R
机构信息
Stem Cell Biology Unit, German Primate Center, Göttingen, Germany.
出版信息
J Med Primatol. 2011 Apr;40(2):111-9. doi: 10.1111/j.1600-0684.2010.00453.x. Epub 2010 Nov 2.
BACKGROUND
Common marmoset monkeys (Callithrix jacchus) are readily used in biomedical research. However, superovulation for embryonic stem cell production and developmental research still remain difficult. Inexplicably, follicle-stimulating hormone (FSH) as key player in superovulation has to be administered in extremely high dosages in this non-human primate compared to human.
METHODS
To evaluate whether marmoset FSH (cjFSH) is functionally more competent than its human homologue on the marmoset FSH receptor (cjFSHR), we established in vitro a homologous system characterizing homologous and recombinantly produced cjFSH.
RESULTS
Upon stimulation of two cell lines stably expressing either the marmoset or the human FSH receptor (cj/hFSHR), respectively, the second messenger signaling of the activated receptor displayed no significant difference in ED(50) values. Thermostability of cjFSH was significantly prolonged by roughly 20% on both FSHRs.
CONCLUSION
High FSH dosage in marmoset superovulation cannot be explained by enhanced biopotency of the natural animal's gonadotropin.
背景
普通狨猴（Callithrix jacchus）常用于生物医学研究。然而，用于胚胎干细胞生产和发育研究的超排卵仍然困难重重。令人费解的是，与人类相比，在这种非人灵长类动物中，作为超排卵关键因素的促卵泡激素（FSH）必须以极高的剂量给药。
方法
为了评估狨猴FSH（cjFSH）在狨猴FSH受体（cjFSHR）上的功能是否比其人类同源物更具活性，我们在体外建立了一个同源系统，用于表征同源和重组产生的cjFSH。
结果
分别刺激稳定表达狨猴或人类FSH受体（cj/hFSHR）的两种细胞系时，激活受体的第二信使信号在半数有效剂量（ED50）值上无显著差异。在两种FSH受体上，cjFSH的热稳定性均显著延长约20%。
结论
狨猴超排卵中高剂量FSH不能用天然动物促性腺激素增强的生物活性来解释。
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