三种非人灵长类动物促卵泡激素β亚基cDNA的分子克隆

Molecular cloning of three nonhuman primate follicle stimulating hormone beta-subunit cDNAs.

作者信息

Wolfgang M J, Grendell R L, Golos T G

机构信息

Wisconsin Regional Primate Research Center, University of Wisconsin, Madison 53715-1299, USA.

出版信息

J Med Primatol. 2001 Dec;30(6):299-303. doi: 10.1034/j.1600-0684.2001.300602.x.

DOI:10.1034/j.1600-0684.2001.300602.x

PMID:11990528

Abstract

The follicle stimulating hormone (FSH) beta-subunit cDNAs were cloned and sequenced for an old world primate, the rhesus monkey (Macaca mulatta), and two New World primates, the common marmoset (Callithrix jacchus) and pygmy marmoset (Cebuella pygmaea). The cDNA and predicted amino acid sequences of the rhesus monkey FSH beta-subunit were related most closely to the human FSH beta-subunit (> 96% identity). The common and pygmy marmosets have identical FSH beta-subunit cDNAs, whereas the marmoset FSH beta-subunit diverges from the rhesus and human molecules with less than 93% identity. These results have significance for the implementation of assisted reproductive technologies in the nonhuman primate as well as the evolution of genes encoding reproductive hormones.

摘要

对一种旧世界灵长类动物恒河猴（猕猴属）以及两种新世界灵长类动物普通狨猴（狨属）和倭狨（倭狨属）的促卵泡激素（FSH）β亚基cDNA进行了克隆和测序。恒河猴FSHβ亚基的cDNA和预测的氨基酸序列与人类FSHβ亚基的关系最为密切（同一性>96%）。普通狨猴和倭狨具有相同的FSHβ亚基cDNA，而狨猴FSHβ亚基与恒河猴和人类分子的差异较大，同一性低于93%。这些结果对于在非人灵长类动物中实施辅助生殖技术以及编码生殖激素的基因的进化具有重要意义。