Institute of Protein Biochemistry, CNR, Via P. Castellino 111, 80131 Naples, Italy.
Fish Shellfish Immunol. 2011 Nov;31(5):688-96. doi: 10.1016/j.fsi.2010.10.018. Epub 2010 Oct 31.
We have investigated the immunoglobulin molecule and the genes encoding it in teleosts living in the Antarctic seas at the constant temperature of -1.86 °C. The majority of Antarctic teleosts belong to the suborder Notothenioidei (Perciformes), which includes only a few non-Antarctic species. Twenty-one Antarctic and two non-Antarctic Notothenioid species were included in our studies. We sequenced immunoglobulin light chains in two species and μ heavy chains, partially or totally, in twenty species. In the case of heavy chain, genomic DNA and the cDNA encoding the secreted and the membrane form were analyzed. From one species, Trematomus bernacchii, a spleen cDNA library was constructed to evaluate the diversity of VH gene segments. T. bernacchii IgM, purified from the serum and bile, was characterized. Homology Modelling and Molecular Dynamics were used to determine the molecular structure of T. bernacchii and Chionodraco hamatus immunoglobulin domains. This paper sums up the previous results and broadens them with the addition of unpublished data.
我们研究了生活在南极海域、水温保持在-1.86°C 的鱼类中的免疫球蛋白分子及其编码基因。大多数南极鱼类属于南极鳕鱼亚目（鲈形目），其中只有少数非南极物种。我们的研究包括 21 种南极鳕鱼和 2 种非南极鳕鱼。我们对两种鱼类的免疫球蛋白轻链进行了测序，对 20 种鱼类的 μ 重链进行了部分或完全测序。在重链方面，我们分析了基因组 DNA 和编码分泌型和膜型的 cDNA。我们从一种鱼类——伯氏突吻鳕（ Trematomus bernacchii ）构建了脾脏 cDNA 文库，以评估 VH 基因片段的多样性。我们对从血清和胆汁中纯化的伯氏突吻鳕 IgM 进行了表征。同源建模和分子动力学用于确定伯氏突吻鳕和鳞头南极鳕鱼（ Chionodraco hamatus ）免疫球蛋白结构域的分子结构。本文总结了以前的结果，并通过添加未发表的数据对其进行了扩展。
