Bretaud Sandrine, Pagnon-Minot Aurélie, Guillon Emilie, Ruggiero Florence, Le Guellec Dominique
Institut de Biologie et Chimie des Protéines, CNRS UMR 5086, IFR 128, Université de Lyon, Université Lyon 1, 7 passage du Vercors, 69367 Lyon cedex 07, France.
Gene Expr Patterns. 2011 Jan-Feb;11(1-2):129-34. doi: 10.1016/j.gep.2010.10.004. Epub 2010 Oct 31.
In mammals, collagen XV is primarily expressed in skeletal and cardiac muscles, and loss of its expression in mice results in a mild skeletal myopathy. We recently identified Col15a1a, a zebrafish ortholog of the human collagen XV gene which expression was restricted to notochord in embryos. Col15a1a knockdown led to defects in muscle maintenance via Shh signaling. Here we report that zebrafish express a second ortholog Col15a1b. The identification of its complete primary sequence showed that the overall structure of collagen XV is well conserved between vertebrates. Whole mount in situ hybridization and RT-PCR analysis revealed that at 12hpf Col15a1b is mainly expressed in slow muscle cell lineage and in nervous tissues, and, at later stages transcripts are detected in eyes, otic placodes and aortic arches. Based on the expression pattern of col15a1b, sequence alignments and synteny comparisons, we conclude that, contrary to collagen XVa, the zebrafish collagen XVb likely displays the same or similar function than the mammalian orthologs.
在哺乳动物中，胶原蛋白XV主要在骨骼肌和心肌中表达，其在小鼠体内的表达缺失会导致轻度骨骼肌病。我们最近鉴定出Col15a1a，它是人类胶原蛋白XV基因在斑马鱼中的直系同源基因，其表达在胚胎期局限于脊索。敲低Col15a1a会通过Shh信号通路导致肌肉维持缺陷。在此我们报告斑马鱼表达第二个直系同源基因Col15a1b。其完整一级序列的鉴定表明，胶原蛋白XV的整体结构在脊椎动物之间高度保守。整体原位杂交和RT-PCR分析显示，在受精后12小时，Col15a1b主要在慢肌细胞谱系和神经组织中表达，在后期阶段，在眼睛、耳基板和主动脉弓中检测到转录本。基于col15a1b的表达模式、序列比对和同线性比较，我们得出结论，与胶原蛋白XVa相反，斑马鱼胶原蛋白XVb可能具有与哺乳动物直系同源基因相同或相似的功能。
