Yu D F, Hu B H, Chen G P, Yang C X, Yang J, Xu J Y, Li L Z
Wuhan General Hospital.
Yao Xue Xue Bao. 1990;25(12):929-31.
A diterpenoid-lactone, white thin crystals, C20H24O3, m/z: 312 (M+), mp 222-223 degrees C, UV lambda max (EtOH) 217 (log epsilon 4.36) nm, has been isolated from the ethyl acetate extract of the roots of Tripterygium wilfordii Hook. f., in a yield of 0.025%. Its structure was elucidated by spectral analysis (UV, IR, MS, 1HNMR and 13CNMR) and X-ray SCD. It is the known triptophenolide with revision of structure. Triptophenlolide was shown to have obvious inhibiting effects on lymphocyte and IgG (P less than 0.01) when mice and rats were given ig 1.5 mg/kg. The total complements in blood serum was increased. When BALB/C mice were given ig 1.5 mg/kg, the ear oedema induced by dimethyl benzene was significantly inhibited (P less than 0.01); The ear oedema induced by croton oil in SD rats at a dose of ig 1.0 mg/kg was also significantly inhibited (P less than 0.05). The vitamin C content of the adrenal gland was reduced in mice at a dose of 1.5 mg/kg. The ig LD50 of triptophenolide was greater than 30 mg/kg.
从雷公藤（Tripterygium wilfordii Hook. f.）根的乙酸乙酯提取物中分离得到一种二萜内酯，为白色细晶体，化学式为C20H24O3，质荷比：312（M+），熔点222 - 223℃，紫外最大吸收波长（乙醇中）217（logε4.36）nm，产率为0.025%。通过光谱分析（紫外、红外、质谱、1H核磁共振和13C核磁共振）及X射线单晶衍射确定其结构。它是已知的雷公藤酚内酯，结构有所修正。给小鼠和大鼠灌胃1.5mg/kg雷公藤酚内酯后，结果显示其对淋巴细胞和IgG有明显抑制作用（P < 0.01），血清总补体增加。给BALB/C小鼠灌胃1.5mg/kg时，对二甲苯所致耳肿胀有显著抑制作用（P < 0.01）；给SD大鼠灌胃1.0mg/kg时，对巴豆油所致耳肿胀也有显著抑制作用（P < 0.05）。给小鼠灌胃1.5mg/kg剂量时，肾上腺维生素C含量降低。雷公藤酚内酯的灌胃半数致死量大于30mg/kg。
