Satomura Takenori, Sakuraba Haruhiko, Hara Yusuke, Ohshima Toshihisa
Department of Materials Science, Yonago National College of Technology, 4448 Hikona-cho, Yonago, Tottori 683-8506, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Nov 1;66(Pt 11):1508-10. doi: 10.1107/S1744309110036808. Epub 2010 Oct 29.
A novel dye-linked L-proline dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix was crystallized using the sitting-drop vapour-diffusion method with polyethylene glycol 8000 as the precipitant. The crystals belonged to the tetragonal space group P4(1)2(1)2 or its enantiomorph P4(3)2(1)2, with unit-cell parameters a = b = 61.1, c = 276.3 Å, and diffracted to 2.87 Å resolution using a Cu Kα rotating-anode generator with an R-AXIS VII detector. The asymmetric unit contained one protein molecule, giving a crystal volume per enzyme mass (V(M)) of 2.75 Å(3) Da(-1) and a solvent content of 55.3%.
利用坐滴气相扩散法，以聚乙二醇8000作为沉淀剂，对来自嗜热需氧古菌——火球菌的一种新型染料连接的L-脯氨酸脱氢酶进行了结晶。晶体属于四方晶系空间群P4(1)2(1)2或其对映体P4(3)2(1)2，晶胞参数a = b = 61.1，c = 276.3 Å，使用配备R-AXIS VII探测器的Cu Kα旋转阳极发生器，晶体衍射至2.87 Å分辨率。不对称单元包含一个蛋白质分子，每个酶质量的晶体体积（V(M)）为2.75 Å(3) Da(-1)，溶剂含量为55.3%。
