应用蛋白 b 引物聚合酶链反应快速诊断结核性脑膜炎的评价。
Evaluation of polymerase chain reaction using protein b primers for rapid diagnosis of tuberculous meningitis.
机构信息
Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
出版信息
Neurol India. 2010 Sep-Oct;58(5):727-31. doi: 10.4103/0028-3886.72189.
BACKGROUND
Rapid and specific diagnosis of tubercular meningitis (TBM) is of utmost importance.
AIM
To evaluate polymerase chain reaction (PCR) using protein b primers directed against M. tuberculosis for the diagnosis of tuberculous meningitis.
MATERIALS AND METHODS
PCR using protein b primers was performed in ten patients with confirmed TBM (culture positive), 60 patients with clinically suspected TBM and 40 patients with no TBM (control group).
RESULTS
Protein b PCR had a sensitivity of 90% and a specificity of 100% in patients with confirmed TBM. In 60 clinically diagnosed TBM patients, protein b PCR was positive in 49 (81.7%) patients. The overall sensitivity of microscopy, culture and PCR using protein b primers was 1.4%, 14.3%, and 82.8% and specificity was 100%, 100%, and 100% respectively.
CONCLUSION
Protein b PCR is valuable in rapid diagnosis of TBM.
背景
快速而特异性地诊断结核性脑膜炎（TBM）至关重要。
目的
评估针对结核分枝杆菌的蛋白 b 引物聚合酶链反应（PCR）在诊断结核性脑膜炎中的应用。
材料与方法
对 10 例确诊的结核性脑膜炎患者（培养阳性）、60 例临床疑似结核性脑膜炎患者和 40 例无结核性脑膜炎患者（对照组）进行蛋白 b 引物 PCR。
结果
在确诊的结核性脑膜炎患者中，蛋白 b PCR 的灵敏度为 90%，特异性为 100%。在 60 例临床诊断为结核性脑膜炎的患者中，蛋白 b PCR 阳性者为 49 例（81.7%）。显微镜检查、培养和蛋白 b 引物 PCR 的总灵敏度分别为 1.4%、14.3%和 82.8%，特异性分别为 100%、100%和 100%。
结论
蛋白 b PCR 对结核性脑膜炎的快速诊断具有重要价值。
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