CNRS UPR4301, Centre de Biophysique Moléculaire, Orléans cedex, France.
RNA Biol. 2010 Nov-Dec;7(6):655-66. doi: 10.4161/rna.7.6.13570. Epub 2010 Nov 1.
The unwinding of RNA helices and the disruption of RNA-protein complexes are critical steps of cellular metabolism that are carried out by ubiquitous NTP-dependent enzymes named RNA helicases. Here, we review the structures, mechanisms, and biochemical properties of two RNA helicases known to adopt a homo-hexameric ring architecture: the P4 packaging motor of bacteriophage φ8, a Super-Family 4 helicase, and Escherichia coli's transcription termination factor Rho from the Super-Family 5 of helicases. We emphasize the many similarities as well as key differences that characterize the Rho and P4 motor mechanisms and highlight important questions that remain to be addressed.
RNA 螺旋的解旋和 RNA-蛋白质复合物的破坏是细胞代谢的关键步骤，这些过程是由普遍存在的、依赖 NTP 的酶（称为 RNA 解旋酶）完成的。在这里，我们回顾了两种已知采用同型六聚体环结构的 RNA 解旋酶的结构、机制和生化特性：噬菌体 φ8 的 P4 包装马达，属于 Super-Family 4 解旋酶，以及 Escherichia coli 的转录终止因子 Rho，属于 Super-Family 5 解旋酶。我们强调了 Rho 和 P4 马达机制的许多相似之处和关键差异，并突出了仍然需要解决的重要问题。
