Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.
Pediatr Res. 2011 Feb;69(2):160-4. doi: 10.1203/PDR.0b013e3182042a07.
Morbidity in the premature (PT) infant may reflect difficult adaptation to oxygen. We hypothesized that feeding including formula feeding (F) and feeding mother's milk (HM) with added fortifier would affect redox status. Therefore, 65 PT infants (birth weight: 1146 ± 261 g; GA: 29 ± 2.5 wk; mean ± SD) were followed biweekly, once oral feeds were introduced. Feeding groups: F (>75% total feeds) and HM (>75% total feeds) were further subdivided according to human milk fortifier (HMF) content of 0-19, 20-49, and ≥ 50%. Oxidative stress was quantified by F2-isoprostanes (F2-IsoPs) in urine, protein carbonyls, and oxygen radical absorbance capacity (ORAC) in plasma. F2-IsoPs (ng/mg creatinine): 0-2 wk, 125 ± 63; 3-4 wk, 191 ± 171; 5-6 wk, 172 ± 83; 7-8 wk, 211 ± 149; 9-10 wk, 222 ± 121; and >10 wk, 183 ± 67. Protein carbonyls from highest [2.41 ± 0.75 (n = 9)] and lowest [2.25 ± 0.89 (n = 12) pmol/μg protein] isoprostane groups did not differ. ORAC: baseline, 6778 ± 1093; discharge, 6639 ± 735 [full term 4 and 12 M, 9010 ± 600 mg (n = 12) TE]. Highest isoprostane values occurred in infants with >50% of their mother's milk fortified. Further research on HMF is warranted.
早产儿（PT）的发病率可能反映了其对氧气适应困难。我们假设，包括配方奶喂养（F）和添加强化剂的母乳（HM）喂养在内的喂养方式会影响氧化还原状态。因此，我们对 65 名早产儿（出生体重：1146 ± 261g；GA：29 ± 2.5 周；均值 ± 标准差）进行了随访，一旦开始口服喂养，每两周进行一次。喂养组：F（>75%总喂养量）和 HM（>75%总喂养量）根据人乳强化剂（HMF）的含量进一步细分为 0-19、20-49 和≥50%。通过尿液中的 F2-异前列腺素（F2-IsoPs）、血浆中的蛋白质羰基和氧自由基吸收能力（ORAC）来量化氧化应激。F2-IsoPs（ng/mg 肌酐）：0-2 周，125 ± 63；3-4 周，191 ± 171；5-6 周，172 ± 83；7-8 周，211 ± 149；9-10 周，222 ± 121；>10 周，183 ± 67。最高 [2.41 ± 0.75（n = 9）]和最低 [2.25 ± 0.89（n = 12）pmol/μg 蛋白] 异前列腺素组的蛋白质羰基无差异。ORAC：基线，6778 ± 1093；出院时，6639 ± 735[足月 4 个月和 12 个月时，9010 ± 600mg（n = 12）TE]。HMF 含量最高的异前列腺素值出现在其母亲的牛奶强化量>50%的婴儿中。需要进一步研究 HMF。
