INRA, UMR 1145, Groupe de gastronomie moléculaire, 16 rue Claude Bernard, 75005 Paris, France.
Anal Bioanal Chem. 2011 Jan;399(1):483-7. doi: 10.1007/s00216-010-4311-6. Epub 2010 Oct 28.
In order to determine the saccharide content of plant tissues, we studied a new non-destructive and fast analytical method that we call "direct quantitative proton nuclear magnetic resonance spectroscopy" (d q (1)H NMR): the application of quantitative proton nuclear magnetic resonance spectroscopy (q (1)H NMR) to non modified plant tissues along with capillary tubes containing a reference compound (for quantification) and deuterium oxide (for locking). Using this method, the saccharide content of samples of carrot (Daucus carota L.) roots was compared to the results given from similar samples by the formerly published q (1)H NMR determination of extracts obtained by the O'Donoghue/Davis method. The content in glucose and sucrose is significantly higher with the direct method than when an extraction step is included; the content in fructose is not significantly different. If a possible detection of saccharides included in glycosylated biological compounds is to be excluded, a more complete extraction of saccharides is probably obtained using the direct method.
为了测定植物组织的糖含量，我们研究了一种新的非破坏性和快速分析方法，我们称之为“直接定量质子核磁共振光谱法”（d q (1)H NMR）：将定量质子核磁共振光谱法（q (1)H NMR）应用于未经修饰的植物组织以及含有参考化合物（用于定量）和重水（用于锁定）的毛细管中。使用这种方法，我们比较了胡萝卜（Daucus carota L.）根的样品的糖含量与以前发表的通过 O'Donoghue/Davis 方法获得的提取物的 q (1)H NMR 测定结果。与包括提取步骤的方法相比，直接方法得到的葡萄糖和蔗糖含量明显更高；果糖含量没有显著差异。如果要排除对糖基化生物化合物中包含的糖的可能检测，则使用直接方法可能会获得更完全的糖提取。
