INRA, UMR 1145, Group of Molecular Gastronomy, 16 rue Claude Bernard, 75005 Paris, France; AgroParisTech, Laboratoire de chimie analytique, Group of Molecular Gastronomy, 16 rue Claude Bernard, 75005 Paris, France; AliXan Inc, France.
INRA, UMR 1145, Group of Molecular Gastronomy, 16 rue Claude Bernard, 75005 Paris, France; AgroParisTech, Laboratoire de chimie analytique, Group of Molecular Gastronomy, 16 rue Claude Bernard, 75005 Paris, France.
Talanta. 2015 Jan;131:335-41. doi: 10.1016/j.talanta.2014.07.097. Epub 2014 Aug 13.
In order to explore the performance of the analytical method called in situ quantitative nuclear magnetic resonance spectroscopy - is q NMR - the distribution of glucose, fructose and sucrose in various parts of a carrot root (Daucus carota L.) - primary xylem, secondary xylem, phloem, cortex; top part and lower part - was determined. The influence on the quality of spectra of drying samples before analysis was studied, as well as the influence of the length of strips of tissue used in analysis. Finally samples as small as 240 mm(3) could be studied directly, with minimum prior treatment (only drying), along with deuterated water for locking and a sealed capillary tube containing a solution of 0.5% of the sodium salt of (trimethylsilyl)propionic-2,2,3,3-d4 acid, used both as an internal reference and for quantification. With optimized parameters, the coefficients of variation for measurements were observed to have an average value of 0.038, with a standard deviation of 0.047.
为了探索原位定量核磁共振光谱分析方法（即 q NMR）的性能，我们对胡萝卜根（Daucus carota L.）的不同部位（初生木质部、次生木质部、韧皮部、皮层；上部和下部）中的葡萄糖、果糖和蔗糖的分布进行了测定。研究了分析前干燥样品对光谱质量的影响，以及分析中所使用的组织条带长度的影响。最后，我们可以直接研究小至 240 mm(3)的样本，只需最小的预处理（仅干燥），同时使用氘水进行锁定，并使用一个密封的毛细管，其中含有 0.5%的（三甲基硅基）丙-2,2,3,3-d4 酸的钠盐溶液，同时用作内标和定量。通过优化参数，观察到测量的变异系数平均值为 0.038，标准偏差为 0.047。
