Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, College of Aquatic Science and Technology, Shanghai Ocean University, 999 Huchenghuan Road, 201306, Shanghai, People's Republic of China.
Fish Physiol Biochem. 2011 Sep;37(3):433-45. doi: 10.1007/s10695-010-9444-1. Epub 2010 Nov 3.
The effects of different lipids on tissue fatty acid profile and reproductive performance in female rice field eel were investigated in this study. Virgin female eels were fed with six diets containing different lipids (diets FO, LO, SO, PO and PL with fish oil, linseed oil, soybean oil, peanut oil and pork lard, respectively; diet APO with arachidonic acid and peanut oil). The results showed that there were positive correlations between the contents of 18:2n-6, 18:3n-3, arachidonic acid (ARA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the tissues of eels and those of the corresponding fatty acids in their diets. The specific growth rate of eels fed with diet PO was the lowest and significantly lower than that of FO and SO. Gonad of eels fed with diets PO and PL showed hypogonadism. The long chain polyunsaturated fatty acids (LC-PUFA) can be synthesized by eels, but the quantity was not enough to meet their reproduction requirement completely. The fatty acid desaturation, rather than elongation probably was one of the limiting factors. Addition of proper amount of ARA in diet was favorable to the increase of the hatching rate of fertilized eggs, while EPA and DHA in diet were beneficial to the increase of the survival rate of larva. Both n-3PUFA and a suitable n-6/n-3PUFA ratio were necessary for growth and reproduction of eels.
本研究旨在探讨不同脂质对雌性稻水象甲组织脂肪酸组成和繁殖性能的影响。将 Virgin 雌性鳗鱼分别喂食六种不同脂质的饲料（FO、LO、SO、PO 和 PL 饲料分别含有鱼油、亚麻籽油、大豆油、花生油和猪油；APO 饲料含有花生油油和花生四烯酸）。结果表明，鳗鱼组织中 18:2n-6、18:3n-3、花生四烯酸（ARA）、二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的含量与相应脂肪酸在其饮食中的含量呈正相关。投喂 PO 饲料的鳗鱼特定生长率最低，显著低于 FO 和 SO 组。投喂 PO 和 PL 饲料的鳗鱼性腺发育不良。鳗鱼可以合成长链多不饱和脂肪酸（LC-PUFA），但数量不足以完全满足其繁殖需求。脂肪酸的去饱和，而不是延伸，可能是一个限制因素。饲料中添加适量的 ARA 有利于提高受精卵的孵化率，而 EPA 和 DHA 有利于提高幼虫的存活率。n-3PUFA 和适当的 n-6/n-3PUFA 比例对鳗鱼的生长和繁殖都是必要的。
