Yue Huamei, Fu Peng, Deng Haichao, Ruan Rui, Ye Huan, Zhang Chuang, Li Chuangju
Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture and Rural Affairs of China, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Chongqing Fishery Sciences Research Institute, Chongqing 400020, China.
Anim Nutr. 2025 Apr 8;21:341-350. doi: 10.1016/j.aninu.2025.03.005. eCollection 2025 Jun.
Arachidonic acid (ARA) is crucial for the growth, antioxidant capacity, and reproductive performance of fish. This study was conducted to assess the impact of dietary ARA on female rice field eel () broodstocks. A total of 600 eels, averaging 117.96 ± 3.65 g, were randomly distributed into 12 tanks, with each tank containing 50 fish. Four isonitrogenous and isolipidic diets comprising 0, 0.50%, 1.00%, and 1.50% ARA were formulated and fed to experimental fish for 10 weeks. Weight gain rates (WGR) increased significantly in 0.50% and 1.00% ARA groups compared to the control (ANOVA, = 0.001; quadratic, < 0.001). The lowest WGR and hepatosomatic index (HSI) values were observed in the 1.50% ARA group. Ovarian crude lipid content decreased in response to the increase of dietary ARA ( < 0.001). Serum alanine aminotransferase (ALT) activity was significantly reduced in 1.00% and 1.50% ARA groups (linear and quadratic, < 0.01). Compared to the control, dietary addition of ARA up to 1.50% increased hepatic superoxide dismutase activity and decreased malondialdehyde content linearly and quadratically ( < 0.01). The ovarian ARA proportion was significantly increased in dietary ARA groups ( < 0.001). Dietary ARA increased the concentration of serum estradiol (E2), and contents of ovarian prostaglandin E2 (PGE2) and vitellogenin (VTG) ( < 0.05). The mRNA levels of ovarian cytochrome P450 (), luteinizing hormone receptor (), and hepatic vitellogenin (v) were significantly upregulated following ARA treatment ( < 0.05). In summary, dietary ARA supplementation increased the growth performance and ovarian deposition of ARA, and reduced the ovarian crude lipid content of . Dietary ARA may regulate the synthesis of steroid hormones and VTG by enhancing the PGE2 downstream signaling pathway, thereby promoting gonadal development in female broodstock. The optimal dietary ARA supplementation for female broodstock is suggested to be 10.00 g/kg.
花生四烯酸（ARA）对鱼类的生长、抗氧化能力和繁殖性能至关重要。本研究旨在评估日粮ARA对雌性黄鳝亲鱼的影响。总共600尾平均体重为117.96±3.65克的黄鳝被随机分配到12个水族箱中，每个水族箱中有50尾鱼。配制了四种等氮和等脂日粮，分别含0%、0.50%、1.00%和1.50%的ARA，并投喂给实验鱼10周。与对照组相比，0.50%和1.00%ARA组的增重率（WGR）显著增加（方差分析，P = 0.001；二次项，P < 0.001）。在1.50%ARA组中观察到最低的WGR和肝体指数（HSI）值。随着日粮ARA的增加，卵巢粗脂肪含量下降（P < 0.001）。1.00%和1.50%ARA组的血清丙氨酸转氨酶（ALT）活性显著降低（线性和二次项，P < 0.01）。与对照组相比，日粮中添加高达1.50%的ARA可使肝脏超氧化物歧化酶活性线性和二次性增加，丙二醛含量降低（P < 0.01）。日粮ARA组的卵巢ARA比例显著增加（P < 0.001）。日粮ARA增加了血清雌二醇（E2）的浓度，以及卵巢前列腺素E2（PGE2）和卵黄蛋白原（VTG）的含量（P < 0.05）。ARA处理后，卵巢细胞色素P450（）、促黄体生成素受体（）和肝脏卵黄蛋白原（v）的mRNA水平显著上调（P < 0.05）。综上所述，日粮中添加ARA可提高黄鳝的生长性能和ARA在卵巢中的沉积，并降低卵巢粗脂肪含量。日粮ARA可能通过增强PGE2下游信号通路来调节类固醇激素和VTG的合成，从而促进雌性黄鳝亲鱼的性腺发育。建议雌性黄鳝亲鱼的最佳日粮ARA添加量为10.00克/千克。
